rDNA Polymorphisms among Different Strains of Isaria fumosorosea

doi:10.16409/j.cnki.2095-039x.2017.01.013

Abstract

Abstract: Phylogenetic and polymorphism analysis based on ITS-5.8S rDNA sequences of different Isaria fumosorosea strains and related species from all over the world were studied. The phylogenetic analysis showed that I. fumosorosea strains were separated into two completely separated Clades, one was clustered into Clade Ⅰ-1 with 94% bootstrap value, the other was into CladeⅡ-1 with 90% bootstrap value. For determining the true taxonomic status of both Clades, polymorphism of I. fumosorosea strains and I. javanica strains were analyzed. The results revealed that there were eight haplotypes, with monophyletic diversity Hd=0.65, nucleotide diversity Pi=0.04 and two different conserved DNA regions. In conclusion, there is large genetic diversity at the species level or assumed populations among I. fumosorosea, indicating that the differences are beyond species boundaries of I. fumosorosea, the strains in CladeⅠ-1 is true I. fumosorosea, and those in CladeⅡ-1 might be I. javanica.

Key words: Isaria fumosorosea, phylogeny, polymorphism, haplotype

CLC Number:

S476.12

WANG Cheng, CHEN Wanhao, HAN Yanfeng, LIANG Zongqi. rDNA Polymorphisms among Different Strains of Isaria fumosorosea[J]. journal1, 2017, 33(1): 85-93.

References

[1] 景云飞, 张仙红. 昆虫病原真菌玫烟色拟青霉的研究进展[J]. 山西农业科学, 2007, 35(9):19-22.
[2] Shimizu S, Yoshioka H, Matsumoto T. Electrophoretic karyotyping of the entomogenous fungus Paecilomyces fumosoroseus[J]. Letters in Applied Microbiology, 1993, 16(4):183-186.
[3] 徐伟松, 胡美英, 钟国华. 真菌杀虫毒素的研究[J]. 云南农业大学学报, 2005, 20(3):339-342.
[4] 罗科, 张青文, 郑应华. 一株拟青霉代谢产物的杀虫效果及生理活性研究[J]. 生物防治通报, 1992, 8(1):22-25.
[5] Lacey L A, Frutos R, Kaya H K, et al. Insect pathogens as biological control agents:do they have a future?[J]. Biological Control, 2001, 21(3):230-248.
[6] Tigano-Milani M S, Honeycutt R J, Lacey L A, et al. Genetic variability of Paecilomyces fumosoroseus isolates revealed by molecular markers[J]. Journal of Invertebrate Pathology, 1995, 65(3):274-282.
[7] Cantone F A, Vandenberg J D. Intraspecific diversity in Paecilomyces fumosoroseus[J]. Mycological Research, 1998, 102(2):209-215.
[8] Fargues J, Bon M C, Manguin S, et al. Genetic variability among Paecilomyces fumosoroseus isolates from various geographical and host insect origins based on the rDNA-ITS regions[J]. Mycological Research, 2002, 106(9):1066-1074.
[9] Inglis P W, Tigano M S. Identification and taxonomy of some entomopathogenic Paecilomyces spp. (Ascomycota) isolates using rDNA-ITS sequences[J]. Genetics and Molecular Biology, 2006, 29(1):132-136.
[10] D'Alessandro C P, Jones L R, Humber R A, et al. Characterization and phylogeny of Isaria spp. strains (Ascomycota:Hypocreales) using ITS1-5.8S-ITS2 and elongation factor 1-alpha sequences[J]. Journal of Basic Microbiology, 2013, 53(1):1-11.
[11] Diosma G, Romanin D E, Rey-Burusco M F, et al. Yeasts from kefir grains:isolation, identification, and probiotic characterization[J]. World Journal of Microbiology and Biotechnology, 2014, 30(1):43-53.
[12] Thompson J D, Gibson T J, Plewniak F, et al. The CLUSTALX windows interface:flexible strategies for multiple sequence alignment aided by quality analysis tools[J]. Nucleic Acids Research, 1997, 25(24):4876-4882.
[13] Tamura K, Stecher G, Peterson D, et al. MEGA6:molecular evolutionary genetics analysis version 6.0[J]. Molecular Biology and Evolution, 2013, 30(12):2725-2729.
[14] Huson D H, Bryant D. Application of phylogenetic networks in evolutionary studies[J]. Molecular Biology and Evolution, 2006, 23(2):254-267.
[15] Leigh J W, Bryant D. Popart:full-feature software for haplotype network construction[J]. Methods in Ecology and Evolution, 2015, 6(9):1110-1116.
[16] Librado P, Rozas J. DnaSP v5:a software for comprehensive analysis of DNA polymorphism data[J]. Bioinformatics, 2009, 25(11):1451-1452.
[17] Amorim R, Savi D C, Ferreira-Maba L, et al. MAT gene idiomorphs suggest a heterothallic sexual cycle in the citrus pathogen hyllosticta citricarpa[J]. European Journal of Plant Pathology, 2016, 64:1-13.
[18] 梁宗琦, 陈万浩, 梁建东, 等. 一个蚁巢中束梗孢类昆虫病原真菌的多型现象[J]. 菌物学报, 2016, 35(8):906-917.
[19] Tajima F. Evolutionary relationship of DNA sequences in finite populations[J]. Genetics, 1983, 105(2):437-460.
[20] Zietkiewicz E, Rafalski A, Labuda D. Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification[J]. Genomics, 1994, 20(2):176-183.
[21] Neigel J E, Avise J C. Applications of a random walk model to geographic distributions of animal mitochondrial DNA variation[J]. Genetics, 1993, 135(4):1209-1220.
[22] Clement M, Posada D, Crandall K A. TCS:a computer program to estimate gene genealogies[J]. Molecular Ecology, 2000, 9(10):1657-1659.
[23] Wiens J J, Penkrot T A. Delimiting species using DNA and morphological variation and discordant species limits in spiny lizards (Sceloporus)[J]. Systematic Biology, 2002, 51(1):69-91.
[24] Sites J W, Marshall J C. Delimiting species:a renaissance issue in systematic biology[J]. Trends in Ecology and Evolution, 2003, 18(9):462-470.
[25] 叶懿, 罗海玻, 侯一平. 单倍型的分析及其法医学应用[J]. 法医学杂志, 2009, 25(2):133-137.
[26] Avin F A, Bhassu S, Tan Y S, et al. Molecular divergence and species delimitation of the cultivated oyster mushrooms:integration of IGS1 and ITS[J]. The Scientific World Journal, 2014:793414.
[27] Cliften P, Sudarsanam P, Desikan A, et al. Finding functional features in Saccharomyces genomes by phylogenetic footprinting[J]. Science, 2003, 301(5629):71-76.
[28] Vingron M, Brazma A, Coulson R, et al. Integrating sequence, evolution and functional genomics in regulatory genomics[J]. Genome Biology, 2009, 10(1):1-8.