Isolation and Identification of Bacillus amyloliquefaciens ZF522 and Its Control Effect on Ginger Root Rot

doi:10.16409/j.cnki.2095-039x.2025.02.038

Abstract

Abstract: Ginger root rot caused by Pythium aphanidermatum seriously endangers ginger production and brings serious economic losses to ginger production. At present, the stem base diseases of ginger are difficult to control, and the use of chemical agents is easy to remain. The biological control of rhizome diseases is also lack of effective prevention and control products. In this experiment, Bacillus was isolated from the rhizosphere soil of ginger, and a strain with good control effect was screened by in vitro screening of ginger root rot. ZF522 was identified as Bacillus amyloliquefaciens according to its morphological characteristics, physiological and biochemical characteristics and phylogenetic tree analysis. Under the condition of pot experiment, the control effect of biocontrol strain ZF522 on ginger root rot reached 50.84%. Agarose gel electrophoresis showed that strain ZF522 contained srfA, fenA, ituA, ituC, bymA, bymB and other antimicrobial lipopeptide synthesis genes. These genes are closely related to the reported antimicrobial lipopeptides. In summary, strain ZF522 has good biocontrol potential against ginger root rot, and is a biocontrol strain with development and utilization value.

Key words: Bacillus amyloliquefaciens, ginger rhizome rot, antagonistic bacterium, strain identification, control effect

CLC Number:

S476

LI Sen, LI Lei, LIU Shicheng, DUAN Guangrong, CHEN Zhukan, SHI Yanxia, CHAI Ali, LI Baoju, XIE Xuewen. Isolation and Identification of Bacillus amyloliquefaciens ZF522 and Its Control Effect on Ginger Root Rot[J]. Chinese Journal of Biological Control, 2025, 41(3): 585-592.

References

[1] Stirling G Ｒ, Turaganivalu U, Stirling A M, et al. Rhizome rot of ginger (Zingiber officinale) caused by Pythium myriotylum in Fiji and Australia[J]. Australasian Plant Pathology, 2009, 38(5): 453-460.
[2] 龙艳艳, 刘增亮, 汪茜, 等. 广西东南沿海生姜茎腐病病原菌鉴定[J]. 西南农业学报, 2017, 30(3): 584-589.
[3] 姜子德, 戚佩坤. 腐霉对姜根茎腐烂的作用[J]. 华南农业大学学报, 1992, 13(4): 97-101.
[4] 元菊丹. 山东生姜茎基腐病病原鉴定及防治研究[D]. 曲阜: 曲阜师范大学, 2011.
[5] 胡鲜梅. 生姜茎基腐病病原菌的鉴定与防治[D]. 泰安: 山东农业大学, 2017.
[6] Ichitani T, Shinsu T. Pythium zingiberum causing rhizome rot of ginger plant and its distribution[J]. Japanese Journal of Phytopathology, 1980, 46(4): 435-441.
[7] Ray M, Dash S, Achary K G, et al. Development and evaluation of polyclonal antibodies for detection of Pythium aphanidermatum and Fusarium oxysporum in ginger[J]. Food and Agricultural Immunology, 2018, 29(1): 204-215.
[8] Athawale V, Paralikar P, Ingle A P, et al. Biogenically engineered nanoparticles inhibit Fusarium oxysporum causing soft-rot of ginger[J]. IET Nanobiotechnology, 2018, 12(8): 1084-1089.
[9] 王翠翠, 杜秉海, 姚良同, 等. 生姜根际茎腐病拮抗菌的筛选及鉴定[J]. 微生物学通报, 2010, 37(12): 1767-1770.
[10] 徐莹莹, 杜秉海, 丁延芹, 等. 生姜根际姜瘟病拮抗菌的筛选及鉴定[J]. 山东农业科学, 2012, 44(1): 79-83.
[11] 李明通. 生姜根腐病的病原菌分离鉴定及其生物防治[D]. 南京: 南京农业大学, 2020.
[12] 东秀珠, 蔡妙英. 常见细菌系统鉴定手册[M]. 北京科学出版社, 2001, 349-418.
[13] Lalucat J, Mulet M, Gomila M, et al. Genomics in bacterial taxonomy: impact on the genus Pseudomonas[J]. Genes, 2020, 11(2): 139.
[14] 师慧娟. 生姜茎基腐病病原微生物的致病机理及抗病效果研究[D]. 济南: 济南大学, 2023.
[15] 刘振伟, 史秀娟. 莱芜生姜茎腐病的病原鉴定[J]. 中国植保导刊, 2009, 29(7): 9-11。
[16] 胡鲜梅. 生姜茎基腐病病原菌的鉴定与防治[D]. 泰安: 山东农业大学, 2017.
[17] Tambong J T, Höfte M. Phenazines are involved in biocontrol of Pythium myriotylum on cocoyam by Pseudomonas aeruginosa PNA1[J]European Journal of Plant Pathology, 2001, 107: 511-521
[18] Zouari I, Jlaiel L, Tounsi S, et al. Biocontrol activity of the endophytic Bacillus amyloliquefaciens strain CEIZ-11 against Pythium aphanidermatum and purification of its bioactive compounds[J]. Biological Control, 2016, 100: 54-62.
[19] Wu Y B， Zhou L B， Lu F X, et al. Discovery of a novel antimicrobial lipopeptide, brevibacillin V, from Brevibacillus laterosporus fmb70 and its application on the preservation of skim milk[J]. Journal of Agricultural and Food Chemistry, 2019, 67(45): 12452-12460.