Screening, Identification and Active Substance Analysis of Biocontrol Bacterium D3-1 against Tobacco PVY

doi:10.16409/j.cnki.2095-039x.2024.02.020

Abstract

Abstract: Viral diseases caused by potato virus Y (PVY) occur extensively in tobacco planting areas, significantly impacting tobacco quality and yield. Through half-leaf necrosis spot method, the biocontrol bacterium D3-1 with excellent efficacy against PVY in tobacco was screened, exhibiting an inhibition rate of 99.71%. Based on morphological, physiological, biochemical characteristics and 16S rDNA sequence analysis, D3-1 was identified to be Bacillus amyloliquefaciens. D3-1 strain displayed broad-spectrum anti antimicrobial activity, inhibiting the growth of pathogenic fungi and bacteria. Field trials with D3-1 fermentation broth (1×10⁹ CFU/mL) resulted in an efficacy of 63.67% against PVY in tobacco. Whole-genome sequencing revealed that strain D3-1 harbored 12 gene clusters responsible for the synthesis of secondary metabolites, including surfactin, fengycin, and bacillibactin, known for their antimicrobial properties. Qualitative analysis using HPLC-MS confirmed the presence of 32 peptide extracts, including surfactin and fengycin. Strain D3-1 exhibited promising efficacy against PVY, demonstrating its potential as a biocontrol agent for managing PVY in tobacco.

Key words: PVY, Bacillus amyloliquefaciens, whole-genome sequencing, gene cluster of secondary metabolite synthesis, active substance

CLC Number:

S476

LI Lingling, CHEN Yibing, TENG Kai, XIAO Zhipeng, ZHOU Xiangping, XIAO Yansong, CAI Hailin, DONG Peng, MEI Siyi, LIU Suoni, WU Shaolong, LIU Tianbo, TANG Qianjun. Screening, Identification and Active Substance Analysis of Biocontrol Bacterium D3-1 against Tobacco PVY[J]. Chinese Journal of Biological Control, 2024, 40(3): 630-640.

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