Effect of Bacillus amyloliquefaciens YN-J3 on Spore Germination and Appressorium Formation of Colletotrichum cereale

doi:10.16409/j.cnki.2095-039x.2024.02.034

Abstract

Abstract: Oat anthracnose is a severe crop disease caused by Colletotrichum cereale, which can damage the aboveground parts of plants. In this study, the antifungal mechanism of B. amyloliquefaciens YN-J3 against C. cereale was investigated. Conidia of C. cereale were treated with strain YN-J3 metabolic fluid, bacterial suspension, metabolites, and cultured plate-to-plate, and the conidia germination rates and appressoria formation rates were determined. The results indicated that the abnormal appressoria formed by the conidia of C. cereale increased in size and lacked melanin deposition when treated directly with the metabolic liquid of the strain YN-J3. Transmission electron microscopy results suggested that the cell wall of the abnormal appressoria of C. cereale was loose, the plasma membrane collapsed, the organelles disintegrated, and the cytoplasm vacuolated. The EC₅₀ values of strain YN-J3 suspension against conidia germination and appressoria formation of C. cereale were 1.14× 10⁵ and 1.91× 10⁴ cfu/mL, respectively. The bacterial suspension of the strain YN-J3 and the conidia of C. cereale were separated by 0.22 μm filter membrane, and the proportion of conidia germination and appressoria formation of C. cereale decreased significantly. The proportion of abnormal appressoria formation at 10⁸ bacteria concentration for 30 h was (15.67± 2.08) %. The ethyl acetate extract of the cell metabolite was able to completely inhibit conidial germination and appressorium formation. When conidia were cultured plate-to-plate with strain YN-J3 for 30 h, they did not germinate. These results indicated that the metabolites produced by strain YN-J3 effectively inhibited the germination of conidia and the formation of appressoria in C. cereale, leading to the development of abnormal appressoria. Furthermore, strain YN-J3 was found to inhibit the formation of infection structures in C. cereale.

Key words: Bacillus amyloliquefaciens, metabolite, spore germination, appressorium formation

CLC Number:

S476

ZHANG Baolong, ZHENG Chengzhong, XU Zhenpeng, QUAN wei, YANG Jifeng, DONG Baozhu, ZHOU Hongyou. Effect of Bacillus amyloliquefaciens YN-J3 on Spore Germination and Appressorium Formation of Colletotrichum cereale[J]. Chinese Journal of Biological Control, 2024, 40(5): 1045-1053.

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