Optimization and Evaluation of Artificial Diet with Insect Ingredients for Arma chinensis

doi:10.16409/j.cnki.2095-039x.2025.09.002

Abstract

Abstract: Arma chinensis is a predator that can effectively suppress a wide range of agricultural and forestry insect pests. Mass rearing of A. chinensis at low costs for use as biocontrol agents is of great significance in sustainable pest management. In this study, the content of Antheraea pernyi pupae was added to former insect-free artificial diet formulated using proteomic methods. Over 3 generations of rearing, the predator was smaller and nymphal development was extended significantly in the diet-fed A. chinensis compared to those reared on pupae of A. pernyi. However, adult weight, fecundity and longevity were higher or longer for the diet-fed A. chinensis compared to those reared on former artificial diet formulated using proteomic methods. The A. chinensis reared on diet with 50% (v/v) content of A. pernyi pupae showed an increased fecundity by 231.05% compared to those reared on former artificial diet formulated using proteomic methods and the female and male adults lived as long as 83.6 d and 103.7 d, respectively. The results indicate that the diet with 50% content of A. pernyi pupae is more suitable for rearing A. chinensis compared to those with 75% content of A. pernyi pupae, considering the rearing costs and the biological performance of the predator.

Key words: Arma chinensis, natural enemy insects, artificial diet optimization, mass rearing, biological control

CLC Number:

S476.2

SONG Liuxiao, XU Weihong, XU Jingyang, CONG Yuqiu, BEN Haiyan, LI Bing, WU Huihui, ZOU Deyu. Optimization and Evaluation of Artificial Diet with Insect Ingredients for Arma chinensis[J]. Chinese Journal of Biological Control, 2025, 41(4): 769-779.

References

[1] Wittmeyer L J, Coudron T A. Life table parameters, reproductive rate, intrinsic rate of increase, and estimated cost of rearing Podisus maculiventris (Heteroptera: Pentatomidae) on an artificial diet[J]. Journal of Economic Entomology, 2001, 94(6): 1344-1352.
[2] Cohen C A. Feeding fitness and quality of domesticated and feral predators: effects of long-term rearing on artificial diet[J]. Biological Control, 2000, 17(1): 50-54.
[3] Sarkar S C, Milroy S P, Xu W. Dietary experience alters predatory behavior of two ladybird species on tomato potato psyllid[J]. Insect Science, 2024, 101927.
[4] 欧阳晶颖, 伍绍龙, 周志成. 混合猎物对蠋蝽生长、繁殖和捕食能力的影响[J]. 中国生物防治学报, 2021, 37(5): 1090-1094.
[5] Clercq D P, Degheele D. Quality assessment of the predatory bugs Podisus maculiventris (Say) and Podisus sagitta (Fab.) (Heteroptera: Pentatomidae) after prolonged rearing on a meat-based artificial diet[J]. Biocontrol Science and Technology, 1993, 3(2):133-139.
[6] Clercq D P, Merlevede F, Tirry L. Unnatural prey and artificial diets for rearing Podisus maculiventris (Heteroptera: Pentatomidae)[J]. Biological Control, 1998, 12(2): 137-142.
[7] 谭晓玲, 王甦, 李修炼, 等. 东亚小花蝽人工饲料微胶囊剂型的研制及饲养效果评价[J]. 昆虫学报, 2010, 53(8): 891-900.
[8] 李立, 杨佳妮, 杨桦, 等. 斑翅肩花蝽布丁人工饲料的饲养效果评价[J]. 昆虫学报, 2013, 56(1): 104-110.
[9] Coudron T A, Wittmeyer J, Kim Y. Life history and cost analysis for continuous rearing of Podisus maculiventris (Say) (Heteroptera: Pentatomidae) on a zoophytophagous artificial diet[J]. Journal of Economic Entomology, 2002, 95: 1159-1168.
[10] Zheng L Y. Arma Hahn (Hemiptera: Stinkbugs) in China[J]. Enemies Insect, 1981, 4: 28-32.
[11] Xia P L,Wang B, Xie X F, et al. Effect of temperature on survival and immature development of Arma chinensis[J]. Journal of Asia-Pacific Entomology, 2022, 25(3): 101927.
[12] 刘孝良, 黄静雅, 张禹, 等. 柞蚕综合利用研究进展[J]. 北方蚕业, 2023, 44(3): 7-11, 35.
[13] 李娇娇, 张长华, 易忠经, 等. 三种猎物对蠋蝽生长发育和繁殖的影响[J]. 中国生物防治学报, 2016, 32(5): 553-561.
[14] 肖雪庄, 徐维红, 李海燕, 等. 应用改良人工饲料及昆虫猎物饲喂蠋蝽的成本分析[J]. 南方农业, 2023, 17(9): 186-189, 196.
[15] 邹德玉. 取食无昆虫成分人工饲料蠋蝽的转录组研究及饲养成本分析[D]. 北京: 中国农业科学院, 2013.
[16] Zou D Y, Wu H H, Coudron T A, et al. A meridic diet for continuous rearing of Arma chinensis (Hemiptera: Pentatomidae: Asopinae)[J]. Biological Control, 2013, 67(3): 491-497.
[17] Zou D Y, Coudron T A, Wu H H, et al. Performance and cost comparisons for continuous rearing of Arma chinensis (Hemiptera: Pentatomidae: Asopinae) on a zoophytogenous artificial diet and a secondary prey[J]. Journal of Economic Entomology, 2015, 108(2): 454-461.
[18] Zou D Y, Coudron T A, Zhang L S, et al. Performance of Arma chinensis reared on an artificial diet formulated using transcriptomic methods[J]. Bulletin of Entomological Research, 2018, 109(1): 24-33.
[19] Zou D Y, Coudron T A, Wu H H, et al. Differential proteomics analysis unraveled mechanisms of Arma chinensis responding to improved artificial diet[J]. Insects, 2022, 13(7): 605-605.
[20] Zou D Y, Coudron T A, Liu C X, et al. Nutrigenomics in Arma chinensis: transcriptome analysis of Arma chinensis fed on artificial diet and Chinese oak silk moth Antheraea pernyi pupae[J]. PLoS ONE, 2013, 8(4): e60881.
[21] 张健, 周毓麟, 孙守慧. 利用人工饲料连代饲养蠋蝽若虫效果评价[J]. 中国森林病虫, 2017, 36(4): 37-40.
[22] 李兴鹏, 宋丽文, 陈越渠, 等. 不同脂肪源人工饲料对蠋蝽生长发育及生殖力的影响[J]. 林业科学, 2018, 54(6): 85-93.
[23] 戴文昊, 刘明宇, 苍宇, 等. 柞蚕蛹胶囊饲料及不同性比对饲养蠋蝽的影响[J]. 中国森林病虫, 2019, 38(2): 17-21.
[24] 段佳辰. 方斑瓢虫人工饲养研究及控害功能评价[D]. 乌鲁木齐: 新疆农业大学, 2022.
[25] 魏帅, 马静, 魏益民, 等. 铅在五指山猪组织器官中的分布与积累研究[J]. 环境科学学报, 2009, 29(10): 2157-2162.
[26] 刘桂荣. 扇贝中重金属残留及食用风险分析[D]. 青岛: 中国海洋大学, 2005.
[27] 周小丽, 余裕娟, 余优军, 等. 惠州市售猪肉和猪肝中重金属元素残留的抽检检测报告分析[J]. 现代食品科技, 2012, 28(3): 342-344.
[28] 戴文昊. 蠋蝽对枸杞棉蚜的捕食功能及其人工饲料的改进研究[D]. 呼和浩特: 内蒙古农业大学, 2019.
[29] Tan X L, Zhao J, Wang S, et al. Optimization and evaluation of microencapsulated artificial diet for mass rearing the predatory ladybird Propylea japonica (Coleoptera: Coccinellidae)[J]. Insect Science, 2015, 22(1): 111-120.
[30] Lapointe S L, Evens T J, Niedz R P. Insect diets as mixtures: optimization for a polyphagous weevil[J]. Journal of Insect Physiology, 2008, 54: 1157-1167.
[31] Huynh M P, Hibbard B E, Lapointe S L, et al. Multidimensional approach to formulating a specialized diet for northern corn rootworm[J]. Scientific Reports, 2019, 9(1): 3709.
[32] Lapointe S L, Evens T J, Niedz R P, et al. Artificial diet optimized to produce normative adults of Diaprepes abbreviatus (Coleoptera: Curculionidae)[J]. Environmental Entomology, 2010, 39(2): 670-677.
[33] Huynh M P, Hibbard B E, Vella M, et al. Development of an improved diet for western corn rootworm using response surface modeling[J]. Scientific Reports, 2019, 9(1): 16009.
[34] Morales-Ramos J A, Rojas M G, Kelstrup H C, et al. Self-selection of agricultural by-products and food ingredients by Tenebrio molitor (Coleoptera: Tenebrionidae) and impact on food utilization and nutrient intake[J]. Insects, 2020, 11(12): 827.
[35] Morales-Ramos J A, Rojas M G, Dossey A T, et al. Self-selection of food ingredients and agricultural by-products by the house cricket, Acheta domesticus (Orthoptera: Gryllidae): A holistic approach to develop optimized diets[J]. PLoS ONE, 2020, 15(1): e0227400.
[36] Özdemir V, Kolker E. Precision nutrition 4.0: A big data and ethics foresight analysis-convergence of agrigenomics, nutrigenomics, nutriproteomics, and nutrimetabolomics[J]. OMICS: A Journal of Integrative Biology, 2016, 20: 69-75.