Identification of Bacillus velezensis BV-3 and Its Control Effect on Southern Corn Leaf Blight

doi:10.16409/j.cnki.2095-039x.2025.02.043

Abstract

Abstract: Southern corn leaf blight caused by Bipolaris maydis has been spreading and causing significant damage in corn-growing regions of Fujian Province. To obtain effective biological control bacteria against southern corn leaf blight, various antagonistic bacteria were isolated from corn rhizosphere soil by the plate dilution method and the antibacterial activity of the isolated strains were determined by the plate confrontation method. Then the strains demonstrating strong antagonism were subsequently identified, and their antibacterial spectrum and pot control efficiency were evaluated. The results showed that 25 monospore strains were isolated from the soil, and 3 strains exhibited significant inhibitory effects against B. maydis. The strain BV-3 domonstrated the strongest antagonism with the inhibition zone width of 18.04 mm. Based on morphological, physiological, biochemical characteristics and 16S rDNA phylogenetic analysis, the strain BV-3 was identified as Bacillus velezensis. This strain displayed a broad antibacterial spectrum and showed good inhibitory effect against 5 pathogenic fungi of corn leaf spot disease. In pot experiment, the control efficacies of strain BV-3 against southern corn leaf spot on resistant variety ‘Xin Zhong Yu 801’ and susceptible variety ‘Min tian 986’ varieties reached 73.72%～92.00% and 68.27%～84.62% in 7～14 days, respectively. In conclusion, Bacillus velezensis BV-3 effectively controls southern corn leaf blight and demonstrates significant potential as a biocontrol agent.

Key words: southern corn leaf blight, Bacillus velezensis, screening and identification, biological control

CLC Number:

S476

WU Mengjing, HUANG Peng, ZHANG Jie, ZHENG Luping, YU Deyi, LIN Sheng, WU Zujian, YAO Jinai. Identification of Bacillus velezensis BV-3 and Its Control Effect on Southern Corn Leaf Blight[J]. Chinese Journal of Biological Control, 2025, 41(4): 887-894.

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