Biocontrol Characteristics of Bacillus velezensis YB-1465 and Its Biocontrol Effects on wheat crown rot

doi:10.16409/j.cnki.2095-039x.2025.02.041

Abstract

Abstract: In recent years, wheat crown rot caused by Fusarium pseudograminearum has emerged as a serious soil-borne disease in Huang-Huai wheat region of China. To identify efficient biocontrol strains against this disease， a promising strain, designated YB-1465, was screened through dual-culture assays, characterization of biocontrol traits, and indoor pot experiments. Phylogenetic analysis based on 16S rDNA and gyrA gene sequences identified YB-1465 as Bacillus velezensis. This strain inhibited the mycelial growth of twelve tested phytopathogenic fungi, demonstrating the highest inhibition rates (>60%) against Fusarium pseudograminearum, Botrytis cinerea, Cochliobolus sativus and Fusarium oxysporum f. sp. vasinfectum. Furthermore, B. velezensis YB-1465 exhibited multiple plant growth-promoting traits, including the production of indole-3-acetic acid (IAA), siderophores, and phosphate solubilization, along with the secretion of extracellular enzymes such as cellulase, β-1,3-glucanase, and pectinase. Compared to the untreated control, seed treatment with the YB-1465 suspension achieved 78.8% control efficacy against wheat seedling crown rot in pot trials. This treatment also significantly enhanced seedling growth, increasing fresh weight, plant height and root length by 148.5%, 65.33%, and 93.24%, respectively. These results demonstrate that Bacillus velezensis YB-1465 possesses significant biocontrol potential against wheat crown rot and warrants further development for biocontrol product formulations.

Key words: wheat crown rot, Fusarium pseudograminearum, Bacillus velezensis, biocontrol, growth-promoting

CLC Number:

S476

XU Wen, XIE Xia, LI Pan, DONG Qianqian, SUN Runhong, ZHANG Jie, XIA Mingcong, WU Chao, YANG Lirong. Biocontrol Characteristics of Bacillus velezensis YB-1465 and Its Biocontrol Effects on wheat crown rot[J]. Chinese Journal of Biological Control, 2025, 41(4): 877-886.

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