Screening and Identification of Biocontrol Bacterium and Its Effect on Tobacco Black Shank Disease and Plant Growth Promotion

doi:10.16409/j.cnki.2095-039x.2025.02.048

Abstract

Abstract: Tobacco black shank, caused by Phytophthora nicotianae, is one of the most destructive and widespread soil-borne diseases of tobacco production. In this study, an efficient antagonistic strain, t1.1, was isolated from the rhizosphere soil of tobacco black shank-affected fields in Qujing, Yunnan Province, using the dilution coating plate method and the plate confrontation method. Based on morphological, physiological and biochemical characterization, as well as the phylogenetic trees constructed from the 16S rRNA and gyrB sequences, the strain was identified and named as Lactiplantibacillus plantarum t1.1. Further studies revealed that strain t1.1 producing siderophores, 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity and the ability to solubilize both organic and inorganic phosphates are capable of stimulating plant growth and disease-suppressing capabilities. Pot experiment revealed that the strain t1.1 provided achieved 67.14% a control efficacy of tobacco black shank, significantly surpassing the positive control (58% metalaxyl-mancozeb at 57.14% efficacy). Concurrently, it exhibited significantly plant growth-promoting effects, markedly enhancing tobacco growth metrics including plant height, root length, fresh weight, and leaf surface area. In summary, strain t1.1 demonstrates promising biocontrol effects against tobacco black shank and exhibits notable plant growth-promoting effects. This study enriched the biological strain resources of this disease and provided a theoretical basis support for the control of lactic acid bacteria for tobacco black shank.

Key words: tobacco black shank, Lactobacillus plantarum, biological control, pot experiment

CLC Number:

S476

GUO Jianjie, YI Han, TANG Jiahao, LIU Yanyu, WEI Jinni, YANG Kaer, ZHANG Hongyan, SHEN Naikun, CHEN Bochang. Screening and Identification of Biocontrol Bacterium and Its Effect on Tobacco Black Shank Disease and Plant Growth Promotion[J]. Chinese Journal of Biological Control, 2025, 41(4): 927-938.

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