Preparation and Application of Suspension of 1.2% Chelerythrine Mixed with 0.8% Osthole

doi:10.16409/j.cnki.2095-039x.2025.02.070

Abstract

Abstract: Recently, rice false smut (RFS) has emerged as a main fungal disease affecting rice. It not only impacts the crop yield and quality but also produces toxins that pose a serious threat to food safety. The judicious blending of botanical fungicides can enhance their efficacy and effectively mitigate the development of fungicide resistance. In our study, single-agent antibacterial tests showed that the EC₅₀ value for chelerythrine was 1.17 μg/mL, while 9.28 μg/mL for osthole. When combined at a mass ratio of 6:4, the mixture exhibited an 81.86% inhibition rate at 7 μg/mL, with an EC₅₀ value of 0.02 μg/mL and a synergistic ratio (SR) of 100.49. The selected formulation of chelerythrine mixed with osthole met the quality control standards, exhibiting a generally concentrated particle size distribution (D₉₀=9.683 μm) and dispersing evenly in water. The formulation of chelerythrine mixed with osthole in a suspension preparation demonstrated good biological control effect against RFS, with efficacy of 76.36% at a 100-fold dilution Due to their environmental friendliness, high efficacy, low risk of pathogen resistance and safety to human and livestock, the botanical fungicides have the great potential for further development and application.

Key words: chelerythrine, osthole, suspension concentrate formulation, rice false smut, control effect

CLC Number:

S476

WEI Qinghui, SONG Weifeng, SHI Zhenghao, LI Zhiyong, PAN Yaqing, WANG Fangyuan, ZHANG Ying, LIU Kai. Preparation and Application of Suspension of 1.2% Chelerythrine Mixed with 0.8% Osthole[J]. Chinese Journal of Biological Control, 2025, 41(6): 1412-1420.

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