Development of Pseudomonas protegens ZF509-Based Wettable Powder Formulation and Its Efficacy in Controlling Bacterial blotch of fruit in Melons

doi:10.16409/j.cnki.2095-039x.2026.02.021

Abstract

Abstract: To develop a highly effective biocontrol agent against bacterial fruit blotch(BFB), this study screened a wettable powder formulation with optimal biocompatibility and quality parameters for Pseudomonas protegens ZF509 and evaluated its protective efficacy in potted plants. The optimized formulation comprised: 90.5% ZF509 fermentation broth, 5% precipitated silica carrier, 1% Morwet IP wetting agent, 1% sodium lignosulfonate dispersant, and 5% food-grade wheat starch protectant. The resulting formulation achieved a viable cell count of 3.8×10⁹ cfu/g, and the quality indicators met the national standards. By adding food-grade wheat starch protectant, the heat resistance of the active ingredient was improved, and the survival rate of ZF509 was above 80% at 37 ℃. The pot experiment showed that the 1000-fold dilution of P. protegens ZF509 wettable powder could effectively control bacterial fruit blotch on melons, with a control efficacy of 49.52%, which was better than the control agent thiazole zinc. This study laid a technical foundation for the research and development and application of P. protegens ZF509 wettable powder.

Key words: Pseudomonas protegens, bacterial fruit blotch, wettable powder, control effect

CLC Number:

S476

ZHAO Zixuan, LIU Yang, SHI Yanxia, XIE Xuewen, CHAI Ali, FAN Tengfei, SUN Xianhua, LI Baoju, LI Lei. Development of Pseudomonas protegens ZF509-Based Wettable Powder Formulation and Its Efficacy in Controlling Bacterial blotch of fruit in Melons[J]. Chinese Journal of Biological Control, 2026, 42(3): 660-671.

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References

[1] Burdman S, Walcott R. Acidovorax citrulli:generating basic and applied knowledge to tackle a global threat to the cucurbit industry[J]. Molecular Plant Pathology, 2012, 13(8):805-815.
[2] Latin RX, Hopkins D. Bacterial fruit blotch of watermelon:the hypothetical exam question becomes reality[J]. Plant Disease, 1995, 79(8):761-764.
[3] O'Brien RG, Martin HL. Bacterial blotch of melons caused by strains of Acidovorax avenae subsp. citrulli[J]. Australian Journal of Experimental Agriculture, 1999, 39(4):479-485.
[4] Silveira E B, Mariano R L R, Michereff S J, et al. Variabilidade de isolados de Acidovorax avenae subsp. citrulli provenientes de melo produzido no estado do Rio Grande do Norte[J]. Summa Phytopatológica, 2003, 29(3):255-261.
[5] 赵廷昌,孙福在,王兵万,等.哈密瓜果斑病病原菌鉴定[J].植物病理学报, 2001, 31(4):357-364.
[6] 金岩,张俊杰,吴燕华,等.西瓜细菌性果斑病的发生与病原菌鉴定[J].吉林农业大学学报, 2004(3):263-266.
[7] 阎莎莎,王铁霖,赵廷昌.瓜类细菌性果斑病研究进展[J].植物检疫, 2011, 25(3):71-76.
[8] Fravel D R. Commercialization and implementation of biocontrol[J]. Annual Review of Phytopathology, 2005, 43(1):337-359.
[9] 孔凡玉,卢平,许永峰,等. 20%青枯灵可湿性粉剂防治烟草青枯病药效试验初报[J].中国烟草科学, 2004(1):36-37.
[10] 李姝江,方馨玫,曾艳玲,等.解淀粉芽孢杆菌BA-12可湿性粉剂研制及对核桃根腐病的防治效果[J].中国生物防治学报, 2016, 32(5):619-626.
[11] 王小洁,李士谣,李亚巍,等.猕猴桃软腐病病原菌的分离鉴定及其防治药剂筛选[J].植物保护学报, 2017, 44(5):826-832.
[12] 汪心玉,芦钰,邱艳红,等.荧光假单胞菌2P24防控瓜类果斑病机制初探[J].中国生物防治学报, 2023, 39(3):575-584.
[13] Almario J, Bruto M, Vacheron J, et al. Distribution of 2,4-Diacetylphloroglucinol biosynthetic genes among the Pseudomonas spp. reveals unexpected polyphyletism[J]. Frontiers in Microbiology, 2017, 8:1218.
[14] Keel C, Weller D M, Natsch A, et al. Conservation of the 2,4-diacetylphloroglucinol biosynthesis locus among fluorescent Pseudomonas strains from diverse geographic locations[J]. Applied and Environmental Microbiology, 1996, 62(2):552.
[15] Ma Z, Zhang S, Liang J, et al. Isolation and characterization of a new cyclic lipopeptide orfamide H from Pseudomonas protegens CHA0[J]. Journal of Antibiotics, 2020, 73(3):179-183.
[16] Muzlera A, Sobrero P, Agaras B, et al. Orfamide production in Pseudomonas protegens CHA0T promotes rhizospheric colonization and influences assemblage of the bacterial community of wheat roots in soil[J]. Rhizosphere, 2024, 30:100882.
[17] 赵子璇,李俊辉,杜公福,等.防御假单胞ZF509的分离鉴定及其对甜瓜细菌性果斑病的防治效果研究[J].中国生物防治学报, 2024, 40(03):690-700.
[18] Mariello M, Kim K, Wu K, et al. Recent advances in encapsulation of flexible bioelectronic implants:materials, technologies, and characterization methods[J]. Advanced Materials, 2022, 34(34):e2201129.
[19] Panichikkal J, Puthiyattil N, Raveendran A, et al. Application of encapsulated Bacillus licheniformis supplemented with chitosan nanoparticles and rice starch for the control of Sclerotium rolfsii in Capsicum annuum(L.)seedlings[J]. Current Microbiology, 2021, 78(3):911-919.
[20] 怀燕.水稻稻种相关细菌及其生防种衣剂的研究[D].杭州:浙江大学, 2010.
[21] 王文桥,马志强,张小风,等.植物病原菌对杀菌剂抗性风险评估[J].农药学学报, 2001(1):6-11.
[22] 李成江,王勇,刘玉敏,等.贝莱斯芽孢杆菌分离鉴定及对哈密瓜细菌性果斑病的防治效果[J].应用与环境生物学报, 2025, 31(9):1456-1466.
[23] 贾慧慧,谢心悦,潘园园,等.芽胞杆菌BJ-6的鉴定及对甜瓜细菌性果斑病的防治[J].微生物学报, 2020, 60(5):982-991.
[24] Fan H, Zhang Z, Li Y, et al. Biocontrol of bacterial fruit blotch by Bacillus subtilis 9407 via surfactin-mediated antibacterial activity and colonization[J].Frontiers in Microbiology, 2017, 8:1973.
[25] Santos E R, Gouveia E R, Mariano R L R, et al. Biocontrol of bacterial fruit blotch of melon by bioactive compounds produced by Bacillus spp.[J].Summa Phytopathologica, 2006, 32(4):376-378.
[26] 李乐书,葛艺欣,田艳丽,等.防治瓜类细菌性果斑病(BFB)生物种衣剂的研制[J].农业生物技术学报, 2015, 23(12):1649-1659.
[27] 武芳.瓜类细菌性果斑病的生物防治研究[D].南京:南京农业大学, 2013.
[28] Lax P, Marro N, Agaras B, et al. Biological control of the false root-knot nematode Nacobbus aberrans by Pseudomonas protegens under controlled conditions[J]. Crop Protection, 2013, 52(5):97-102.
[29] Ajijah N, Fiodor A, Dziurzynski M, et al. Biocontrol potential of Pseudomonas protegens ML15 against Botrytis cinerea causing gray mold on postharvest tomato(Solanum lycopersicum var. cerasiforme)[J]. Frontiers in Plant Science, 2023, 14:1288408.
[30] Andreolli M, Zapparoli G, Angelini E, et al. Pseudomonas protegens MP12:A plant growth-promoting endophytic bacterium with broad-spectrum antifungal activity against grapevine phytopathogens[J]. Microbiological Research, 2019, 219:123-131.
[31] Michavila G, Adler C, De Gregorio P R, et al. Pseudomonas protegens CS1 from the lemon phyllosphere as a candidate for citrus canker biocontrol agent[J]. Plant Biology, 2017, 19(4):608-617.
[32] Henkels M D, Kidarsa T A, Shaffer B T, et al. Pseudomonas protegensPf-5 causes discoloration and pitting of mushroom caps due to the production of antifungal metabolites[J]. Molecular Plant-Microbe Interactions, 2014, 27(7):733-746.