Abstract: Sympiezomias citri (Coleoptera: Curculionidae) is one of the most important agricultural pests causing great damage on various economic crops, and chemical control is the usual treatment method for this beetle at present. In order to explore alternative strategy for sustainable control of the pest population, the virulence of 15 strains of Beauveria spp. to S. citri was tested under laboratory condition. The strain Bb2-1 was selected for the most virulent to the adults of S. citri, and caused the highest corrected mortality of 100%, highest muscardine rates of 97.78% and shortest LT₅₀ of 3.77 d after fungal inoculation at a concentration of 1.0×10⁸ spores/mL (7 d, 26 ℃). The LC₅₀ value was 4.55×10⁴ spores/mL at 26 ℃. According to culture characteristics, morphology feature and rDNA ITS sequence analysis, the strain Bb2-1 was identified to be Beauveria bassiana. The effect of five medium types (PDA, PPDA, 1/4 SDAY, SDAY, and Czapek), five temperature gradient (15, 20, 25, 30 and 35 ℃), seven different pH value (pH 4.0—10.0) and seven different illumination periods (0L:24D, 24L:0D, 18L:6D, 12L:12D, 6L:18D, dark in the first 7 days and light in the last 7 days, and light in the first 7 days and dark in the last 7 days) on colony growth and sporulation of the strain Bb2-1 were evaluated through the determination of colony diameter and sporulation. The results showed that SDAY medium at pH 7.0 was the most optimal medium for the colony growth and sporulation of the strain Bb2-1 at 25 ℃, and photoperiod didn't pose any obvious effects on colony growth, but prolonging illumination time was benefit to the sporulation. In this study, the selection of a highly virulent B. bassiana (Bb2-1) towards S. citri and the evaluation of culture characteristics of Bb2-1 would provide reference for large scale production of the fungi to control this beetle.