Abstract: In this study, we tested the acid protease, neutral protease, alkaline protease, chitinase, phospholipase enzyme activities of six Metarhizium anisopliae transformants with cry8E gene insertion and virulence against Holotrichia parallela in order to clarify the correlationship between extracellular enzyme activity and virulence of each strain. The results showed that the virulence among six transformants assayed was significantly different, and the ranges of the estimated LT₅₀ and the corrected cumulative mortality were 14.48～21.26 d, 64.50%～92.86%, respectively. There also has significant difference in the enzyme activities among six transformants. Regression analysis also revealed that acid protease and chitinase were significantly correlated to the corrected cumulative mortality and LT₅₀. Binary regression analysis of combination effects of acid protease and chitinase on virlence also revealed that only chitinase was significantly correlated to the corrected cumulative mortality and LT₅₀. It suggested that the difference of virulence among transformants may be mainly derived from the activity of chitinase, and it could be used as virulence index for large scale of early candidate transformants selection, however, but it could not completely replace the virulence measurement in future.