Abstract: Bacillus thuringiensis FB strain was high toxicity against larvae of Plutella xylostella (L.). FB strain was characterized with scanning electron microscope, large plasmids electrophoresis, SDS-PAGE and growth characteristics observation. Five full length toxin genes, cry1Ia, cry1Ea, cry1Ab, cry2Ab and vip3Aa, were cloned. Furthermore, a half-length fragment of cry8 gene was found by whole-genome sequencing, this is the first time that cry1 and cry8-type genes existed in the same strain. The highest identity of the deduced amino acid sequences with known B. thuringiensis toxins were 99%, 98%, 99%, 100%, 99% respectively, but deduced amino acid sequence of this Cry8 fragment was 63% similarity with known Cry8 proteins. Bioassay results showed that Cry1Ab, Cry1Ia, Cry1Ea were high toxicity against larvae of P. xylostella.