Biological and Ecological Characteristics of a Thermotolerant Strain of Anagrus nilaparvatae

doi:10.16409/j.cnki.2095-039x.2021.01.013

Abstract

Abstract: The parasitoid, Anagrus nilaparvatae Pang et Wang, is one of the major natural enemies of rice planthoppers, while its population dynamics are frequently subjected to the restriction of high temperature. In this study, we measured the developmental duration, effective accumulative temperature, host egg preference, oviposition amount, and stress response parameters at extremely high temperatures of a thermotolerant strain of A. nilaparvatae (NR strain, acclimated at 35℃ for 24 generations) to characterize its biocontrol potential. The results showed that, compared with the A. nilaparvatae control strain HZ, males of the NR strain showed a significantly higher development rate at 20℃, and the difference was mainly due to the egg-middle larvae stage and the pupal stage. NR strain oviposited even at high temperatures, with 18.80 eggs per female at 35℃. NR strain showed higher tolerance to extreme temperatures than HZ strain. The heat knockdown time of NR strain males at 50℃ was 15.19 min, 3.03 min longer than that of the HZ strain. Furthermore, the fecundity of NR strain was affected by nutriments. Supplement of 10% sucrose at 35℃ could not only prolong lifespan but also increase fecundity. Those results indicate that the A. nilaparvatae strain acclimated at high temperatures can maintain normal growth and parasitism at 35℃, which confers its potential to adapt to high temperatures in summer.

Key words: Anagrus nilaparvatae, thermotolerant strain, temperature, developmental duration, fecundity

CLC Number:

S476.3

WANG Bingyang, HUANG Tingfa, MA Ying, TANG Bingjie, ZHOU Qiang, ZHANG Guren. Biological and Ecological Characteristics of a Thermotolerant Strain of Anagrus nilaparvatae[J]. Chinese Journal of Biological Control, 2021, 37(4): 692-700.

References

[1] 包建中, 古德祥, 陶志新, 等. 中国生物防治[M]. 太原:山西科学技术出版社, 1998, 143-144.
[2] Yu X P, Barrion A T, Lu Z X. A taxonomic investigation on egg parasitoid, Anagrus of rice planthopper in Zhejiang Province[J]. Chinese Rice Research Newsletter, 2001, 9(3):8-9.
[3] 毛润乾, 古德祥, 张古忍, 等. 稻田飞虱卵寄生蜂群落结构和动态的初步研究[J]. 昆虫学报, 2002, 45(3):408-412.
[4] 祝增荣, 陈建明, 程家安, 等. 双季稻白背飞虱的被寄生率暨存活率分析[J]. 中国生物防治, 2004(1):21-26.
[5] 郑许松, 俞晓平, 吕仲贤, 等. 不同营养源对稻虱缨小蜂寿命及寄生能力的影响[J]. 应用生态学报, 2003, 14(10):1751-1755.
[6] 林庆胜, 黄寿山, 胡美英, 等. 稻虱缨小蜂(Anagrus nilaparvatae)在两种稻飞虱上的生殖力及其应用潜能[J]. 生态学报, 2009, 29(8):4295-4302.
[7] 朱平阳, 郑许松, 姚晓明, 等. 提高稻飞虱卵期天敌控害能力的稻田生态工程技术[J]. 中国植保导刊, 2015, 35(7):27-32.
[8] 申昭灿, 陈龙, 邬家栋, 等. 稻田寄生蜂和鳞翅目昆虫的多样性及变化动态[J]. 中国生物防治学报, 2017, 33(5):590-596.
[9] Nguyen T M, Bressac C, Chevrier C. Heat stress affects male reproduction in a parasitoid wasp[J]. Journal of Insect Physiology, 2013, 59(3):248-254.
[10] 蒋丰泽, 郑灵燕, 郭技星, 等. 温度对昆虫繁殖力的影响及其生理生化机制[J]. 环境昆虫学报, 2015, 37(3):653-663.
[11] 徐国民, 程遐年. 温度对稻虱缨小蜂实验种群增长的影响[J]. 南京农业大学学报, 1989, 12(1):53-59
[12] 刘淑平, 郑许松, 杨亚军, 等. 温度对稻虱缨小蜂生态适应性的持续影响[J]. 中国生物防治学报, 2012, 28(1):20-26.
[13] 罗肖南, 卓文禧. 稻田飞虱与天敌数量消长关系及其自然控制作用考查[J]. 昆虫天敌, 1986(2):72-79.
[14] 张晓燕, 翟一凡, 林清彩, 等. 寄生蜂对稻飞虱控害作用研究进展[J]. 环境昆虫学报, 2014, 36(6):1025-1032.
[15] 尤珂珂, 袁志能, 颜静, 等. 褐飞虱取食人工饲料的刺吸电位波形鉴别[J]. 环境昆虫学报, 2018, 40(3):536-542.
[16] 丁岩钦. 昆虫数学生态学[M]. 北京:科学出版社, 1994, 318-329.
[17] 李丽英, 张月华, 张荣华. 赤眼蜂生长发育与温度关系的种间及种内差异[J]. 昆虫天敌, 1983(1):1-5.
[18] 祝增荣, 程家安, 陈琇. 温度和食物对稻虱缨小蜂发育、存活和繁殖的影响[J]. 生态学报, 1991(1):66-72.
[19] Gilchrist G W, Huey R B. The direct response of Drosophila melanogaster to selection on knockdown temperature[J]. Heredity, 1999, 8(1):15-29.
[20] Hoffmann A A, Chown S L, Clusella-Trullas S, et al. Upper thermal limits in terrestrial ectotherms:how constrained are they?[J]. Functional Ecology, 2013, 27(4):934-949.
[21] Moseley P L. Heat shock proteins and heat adaptation of the whole organism[J]. Journal of Applied Physiology, 1997, 83(5):1413-1417.
[22] Kevan P G, Baker H G. Insects as flower visitors and pollinators[J]. Annual Review of Entomology, 1983, 28:407-453.
[23] Wäckers F L, Steppuhn A. Characterizing nutritional state and food source use of parasitoids collected in fields with high and low nectar availability[J]. IOBC wprs Bulletin, 2003, 26(4):203-208.
[24] Castellanos N L, Bueno A F, Haddi K, et al. The fitness and economic benefits of rearing the parasitoid Telenomus podisi under fluctuating temperature regime[J]. Neotropical Entomology, 2019, 48(6):934-948.
[25] McCalla K A, Keçeci M, Milosavljević I, et al. The influence of temperature variation on life history parameters and thermal performance curves of Tamarixia radiata (Hymenoptera:Eulophidae), a parasitoid of the Asian citrus psyllid (Hemiptera:Liviidae)[J]. Journal of Economic Entomology, 2019, 112(4):1560-1574.