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Chinese Journal of Biological Control ›› 2024, Vol. 40 ›› Issue (2): 378-387.DOI: 10.16409/j.cnki.2095-039x.2024.02.013

• RESEARCH REPORTS • Previous Articles    

Identification of Active Substances of Bacillus atrophaeus LB6-2 to Inhibit Rhizoctonia solani

LUO Wenfang, ZHOU Junhui, HE Wei, XU Jianjun   

  1. Key Laboratory of Integrated Pest Management on Crops in Northwestern Oasis/Ministry of Agriculture and Rural Affairs, Xinjiang Key Laboratory of Agricultural Bio-safety/Institute of Plant Protection/Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China
  • Received:2023-09-01 Published:2024-04-28

Abstract: Bacteria LB6-2 was selected against Rhizoctonia solani by plate face-off method,mycelium growth rate method and sclerotium germination inhibition method.It showed the inhibition rate of mycelium growth and sclerotium germination of strain LB6-2 was 71.76% and 100.00%.The strain was identified Bacillus atrophaeus(OK639005.1) by observing morphological characteristics,analyzing physicochemical properties and 16S rDNA amplification.The fermentation products were extracted by macroporous resin method and prepared by HPLC.Fourteen active components were determined by activity determination,and the single compounds were prepared and analyzed by HPLC-MS,NMR and activity determination.The compounds LBN4-2,LBN4-4 and LBN4-5 were identified as Cyclo (Pro-Val),Cyclo (D-Leu-L-Pro) and Cyclo (L-prolyl-D-phenylalanyl),respectively.The activity assay of the three compounds showed that the inhibition rates of hyphal growth were 86.16%,84.87% and 83.10% at concentration was 4 mmol/L,respectively.While the concentration of the three compounds were above 2 mmol/L,the inhibition rates of hyphal germination were 100%.In summary,Bacillus atrophaeus LB6-2 has the potential to be used as a biocontrol agent against Rhizoctonia solani.

Key words: Rhizoctonia solani, Bacillus atrophaeus, active substance, structure identification

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