Identification of Bacteria strain Sneb2010 and Its Control Effect on Melon Fusarium Wilt

doi:10.16409/j.cnki.2095-039x.2024.02.063

Abstract

Abstract: Melon Fusarium wilt is a serious soil-borne disease caused by Fusarium oxyporum f. sp. melonis. In this study, the bacteria strain Sneb2010 with significant inhibitory effect on F. oxyporum f. sp. melonis was selected from 688 strains of bacteria by the dual culture technique, and the inhibition rate was 78.45%. The bacterium Sneb2010 was identified as Bacillus velezensis by morphological, physiological and biochemical characteristics combined with 16S rDNA and gyrA sequence analysis. The mycelium growth and spore germination of F. oxyporum f. sp. melonis were significantly inhibited by strain Sneb2010. After Sneb2010 treatment, the mycelia of F. oxyporum f. sp. melonis in the inhibited area showed abnormal phenomena, such as wizened, wrinkled, irregular protrusion and fracture. Strain Sneb2010 could promote the germination of melon seeds and the growth of seedlins. It was found that the strain Sneb2010 could produce protease and had the ability of biofilm forming. In addition, strain Sneb2010 showed strong inhibitory effects on a variety of pathogenic fungi. The results of pot experiment showed that, the control effect of Sneb2010 on melon Fusarium wilt was 64.29%, the number of pathogens decreased by 47%. In conclusion, B. velezensis Sneb2010 could effectively control melon Fusarium wilt, and provide a new potential biocontrol resource for the biological control of melon Fusarium wilt.

Key words: melon Fusarium wilt, Biocontrol bacteria, screen, Identification, Bacillus velezensis

CLC Number:

S476

WU Ji, ZHU Xiaofeng, WANG Yuanyuan, LIU Xiaoyu, ZHAO Di, YANG Ning, DUAN Yuxi, FAN Haiyan, CHEN Lijie. Identification of Bacteria strain Sneb2010 and Its Control Effect on Melon Fusarium Wilt[J]. Chinese Journal of Biological Control, 2024, 40(6): 1331-1346.

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