Efficacy of Bacillus velezensis B9601-Y2 on Leaf Spot Disease of Honeysuckle

doi:10.16409/j.cnki.2095-039x.2024.02.067

Abstract

Abstract: To evaluate the efficacy of Bacillus velezensis strain B9601-Y2 (referred to as Y2) in controlling honeysuckle leaf spot disease, the antagonistic potential of Y2 towards Alternaria alternate, the causative agent of leaf spot disease, was explored through plate confrontation and Oxford cup inhibition assays. The influence of Y2 on the pathogen's mycelial and spore morphology was assessed via centrifuge tube-based co-culture experiments. Furthermore, the capacity of Y2 to inhibit spore germination of the leaf spot pathogen was quantified through spore germination assays. The overall control efficacy of Y2 against leaf spot disease was evaluated through pot experiments. The results revealed that Y2 and its crude lipopeptide extracts demonstrated inhibition rates of 67.36% and 44.93% against the pathogen, respectively. Moreover, both the supernatant and crude lipopeptide extracts of Y2 caused significant swelling of the pathogen's mycelia and spores, and markedly suppressed spore germination. Application of Y2 bacterial suspension on honeysuckle resulted in control efficiencies of 75.65% and 76.06% against leaf spot disease on the 10th and 21st days post-application, respectively. These efficiencies are on par with those of the chemical fungicide 30% pyraclostrobin suspension. These findings highlight the potential of Y2 as a promising biological control agent against honeysuckle leaf spot disease, suggesting its prospects for broader implementation and application in sustainable agriculture.

Key words: honeysuckle leaf spot disease, Bacillus velezensis, biocontrol, antagonistic activity, crude lipopeptide extract

CLC Number:

S476

CUI Wenyan, LUO Xiyan, ZHANG Jiajia, CHE Jingjing, ZOU Yuan, CHEN Kunxiu, HE Pengjie. Efficacy of Bacillus velezensis B9601-Y2 on Leaf Spot Disease of Honeysuckle[J]. Chinese Journal of Biological Control, 2024, 40(6): 1347-1354.

References

[1] 朱文卿, 任汉书, 郑媛媛, 等. 金银花的功能性成分及其生物活性研究进展[J]. 食品工业科技, 2021, 42(13): 412-426.
[2] 汪晓露, 付畅, 赵勇, 等. 中药金银花治疗新型冠状病毒肺炎机制探究[J]. 中药材, 2020, 43(9): 2340-2343.
[3] He P J, Luo X Y, Wu X Y, et al. Leaf spot of Lonicera japonica Thunb.(honeysuckle) caused by Alternaria alternata newly reported in China[J]. Crop Protection, 2023, 170: 106269.
[4] 姚银花, 佀胜利, 郑福山. 药用植物金银花病虫害种类及综合防治[J]. 凯里学院学报, 2008, 26(3): 56-59.
[5] 焦梓洲. 生物防治技术在农业病虫害防治中的应用分析[J]. 种子科技, 2020,38(3): 85-86.
[6] Lahlali R, Ezrari S, Radouane N, et al. Biological control of plant pathogens: A global perspective[J]. Microorganisms, 2022, 10(3): 596.
[7] 邹晓璐, 赵雨, 马东丽, 等. 贝莱斯芽胞杆菌ZJ1油悬剂的研制及其田间防病促生效果[J]. 中国生物防治学报, 2023, 39(5): 1204-1214.
[8] 祝文慧, 秦溥, 赵岩, 等. 两种芽胞杆菌对苗期土传辣椒疫病的防控作用[J]. 中国生物防治学报, 2023, 39(5): 1235-1243.
[9] Fan B, Wang C, Song X F, et al. Bacillus velezensis FZB42 in 2018: the gram-positive model strain for plant growth promotion and biocontrol[J]. Frontiers in microbiology, 2018, 9: 2491.
[10] Pan Z X, Munir S, Li Y M, et al. Deciphering the Bacillus amyloliquefaciens B9601-Y2 as a potential antagonist of tobacco leaf mildew pathogen during flue-curing[J]. Frontiers in Microbiology, 2021, 12: 683365.
[11] Cui W Y, He P J, Munir S, et al. Efficacy of plant growth promoting bacteria Bacillus amyloliquefaciens B9601-Y2 for biocontrol of southern corn leaf blight[J]. Biological Control, 2019, 139: 104080.
[12] 田丹丹, 周维, 李朝生, 等. 香蕉枯萎病拮抗菌GKT04产生的脂肽类抗生素的分离和鉴定[J]. 南方农业学报, 2020, 51(5): 1122-1127.
[13] 崔文艳, 罗喜燕, 黄高, 等. 金银花白粉病生防内生菌株的筛选、鉴定及其对白粉病的防效[J]. 西南农业学报, 2023, 36(5): 958-964.
[14] Gao H Y, Li P Z, Xu X X, et al. Research on volatile organic compounds from Bacillus subtilis CF-3: Biocontrol effects on fruit fungal pathogens and dynamic changes during fermentation[J]. Frontiers in Microbiology, 2018, 9: 456.
[15] Zhang N, Wang Z Q, Shao J H, et al. Biocontrol mechanisms of Bacillus: Improving the efficiency of green agriculture[J]. Microbial Biotechnology, 2023, 16(12): 2250-2263.
[16] 郑爱芳, 张名雪, 周姝珂, 等. 香樟黑斑病病原菌鉴定及其拮抗菌B505的生防效果[J]. 西北农业学报, 2024, 33(6): 1163-1173.
[17] 刘涵斐, 徐婷婷, 李锡宏, 等. 贝莱斯芽孢杆菌F85对烟草炭疽病的生防作用研究[J]. 中国烟草科学, 2023, 44(3): 47-52, 61.
[18] 田凤鸣, 陈强, 何九军, 等. 一株花椒根腐病拮抗菌的筛选、发酵条件及其抑菌物质的初步分析[J]. 微生物学通报, 2023, 50(7): 2950-2969.
[19] Kim J A, Song J S, Kim P I, et al. Bacillus velezensis TSA32-1 as a promising agent for biocontrol of plant pathogenic fungi[J]. Journal of Fungi, 2022, 8(10): 1053.
[20] Gu Q, Yang Y, Yuan Q M, et al. Bacillomycin D produced by Bacillus amyloliquefaciens is involved in the antagonistic interaction with the plant-pathogenic fungus Fusarium graminearum[J]. Applied and Environmental Microbiology, 2017, 83: e01075-17.
[21] He P F, Hao K, Blom J, et al. Genome sequence of the plant growth promoting strain Bacillus amyloliquefaciens subsp. plantarum B9601-Y2 and expression of mersacidin and other secondary metabolites[J]. Journal of biotechnology, 2013, 164(2): 281-291.
[22] He P J, Cui W Y, Peng L. Biocontrol efficacy of Bacillus velezensis HC-8 against powdery mildew of honeysuckle caused by Erysiphe lonicerae var. Lonicerae[J]. Biological Control, 2022, 166: 104834.