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中国生物防治学报 ›› 2017, Vol. 33 ›› Issue (2/3): 165-170.DOI: 10.16409/j.cnki.2095-039x.2017.02.003

• 研究论文 • 上一篇    下一篇

低温处理替代寄主对管氏肿腿蜂繁殖的影响

胡镇杰1,2, 杨海博1, 林晓民1, 张青文2, 董钧锋1   

  1. 1. 河南科技大学林学院, 洛阳 471023;
    2. 中国农业大学植物保护学院, 北京 100193
  • 收稿日期:2016-08-12 出版日期:2017-06-08 发布日期:2017-04-08
  • 通讯作者: 董钧锋,副教授,E-mail:junfengdong@126.com。
  • 作者简介:胡镇杰,博士研究生,讲师,E-mail:zhenjiehu@163.com
  • 基金资助:
    河南科技大学青年基金(2015QN034);河南科技大学大学生创新实践平台(2003-17510002)

Effects of Low Temperature Storage of Substituted Host on the Reproduction of Sclerodermus guani

HU Zhenjie1,2, YANG Haibo1, LIN Xiaomin1, ZHANG Qingwen2, DONG Junfeng1   

  1. 1. College of Forestry, Henan University of Science and Technology, Luoyang 471023, China;
    2. College of Plant Protection, China Agricultural University, Beijing 100193, China
  • Received:2016-08-12 Online:2017-06-08 Published:2017-04-08

摘要: 大规模、低成本繁殖天敌是开展害虫生物防治的前提和保障。本文以天牛类害虫寄生性天敌管氏肿腿蜂为研究对象,研究了-6、-3、0、3和6 ℃处理替代寄主黄粉甲蛹24 h(光周期16L:8D,相对湿度80%±10%)对该蜂人工繁殖的影响。结果表明,低温处理黄粉甲蛹可提升管氏肿腿蜂的繁殖效率,但-6 ℃下黄粉甲蛹的死亡率高达40.00%,不利于寄主资源的高效利用。在设置的5个温度梯度下,以-3 ℃处理黄粉甲蛹时寄主死亡率为16.67%,管氏肿腿蜂寄生率和存活率分别为86.67%和100.00%,寄生蜂麻痹寄主所需时间及管氏肿腿蜂产卵前期分别为9.6 h和4.67 d,后代羽化量为29.30头。低温处理替代寄主提高了管氏肿腿蜂子代幼虫的存活率,以-3 ℃处理更适宜,同时对卵和茧的存活率无显著影响。

关键词: 低温, 替代寄主, 管氏肿腿蜂, 大规模繁殖

Abstract: Large scale and low cost reproduction of natural enemies is the prerequisite and guarantee for biological control of pest insects. Effects of low temperature treatment of the substituted host, pupae of Tenebrio molitor, on the reproduction of parasitoid Sclerodermus guani were determined by exposing T. molitor to-6,-3, 0, 3, or 6℃ for 24 h. Results showed that low temperature treatment of T. molitor pupae could increase the reproduction of S. guani. However, the mortality of host pupae reached 40.00% at-6℃, which is not beneficial for the efficient utilization of host resources. In the treatment of-3℃, the host mortality, parasitism rate and survival rate of S. guani were 16.67%, 86.67% and 100.00% respectively, and duration of paralyzing time, duration of pre-oviposition and amount of adult offspring were 9.6 h, 4.67 d and 29.30, respectively. Low temperature treatment of pupae of T. molitor can increase larval survival of parasitoid and-3℃ is the most suitable treatment temperature; with no obvious negative effects on egg hatching or cocoon survival.

Key words: low temperature, substituted host, Sclerodermus guani, mass rearing

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