香蕉枯萎病拮抗菌贝莱斯芽胞杆菌的筛选鉴定及其生物学特性

doi:10.16409/j.cnki.2095-039x.2021.01.031

中国生物防治学报 ›› 2021, Vol. 37 ›› Issue (1): 165-171.DOI: 10.16409/j.cnki.2095-039x.2021.01.031

• 研究论文 • 上一篇

香蕉枯萎病拮抗菌贝莱斯芽胞杆菌的筛选鉴定及其生物学特性

杨迪^1,2, 杜婵娟², 张晋², 潘连富², 叶云峰³, 黄思良¹, 付岗²

1. 南阳师范学院生命科学与农业工程学院, 南阳 473061;
2. 广西农业科学院植物保护研究所/广西作物病虫害生物学重点实验室, 南宁 530007;
3. 广西农业科学院园艺研究所, 南宁 530007

收稿日期:2020-05-28 发布日期:2021-02-23
通讯作者: 黄思良,博士,教授,E-mail:silianghuang@aliyun.com;付岗,博士,研究员,E-mail:fug110@gxaas.net。
作者简介:杨迪,硕士研究生,研究实习员,E-mail:1464596962@qq.com
基金资助:
广西自然科学基金（2017GXNSFDA198002）；广西创新驱动发展专项（桂科AA18118028-5）；广西农业科学院科技发展基金（桂农科2018YT15，31960520，2020YM96）；广西作物病虫害生物学重点实验室基金（17-259-47-ST-1）

Screening, Identification and Biological Characteristics of Bacillus velezensis with Antagonst Activity against Banana Fusarium Wilt

YANG Di^1,2, DU Chanjuan², ZHANG Jin², PAN Lianfu², YE Yunfeng³, HUANG Siliang¹, FU Gang²

1. School of Lifescience and Agricultural Engineering, Nanyang Normal University, Nanyang 473061, China;
2. Guangxi Key Laboratory of Biology for Crop Diseases and Insect Pests/Institute of Plant Protection Research, Guangxi Academy of Agricultural Sciences, Nanning 530007, China;
3. Institute of Horticulture, Guangxi Academy of Agricultural Sciences, Nanning 530007, China

Received:2020-05-28 Published:2021-02-23

摘要/Abstract

摘要： 香蕉枯萎病是目前香蕉产业面临的毁灭性病害，目前尚无特别有效的防治药剂。为发掘香蕉枯萎病的生防菌资源，本研究通过初筛和复筛获得两株对香蕉枯萎病菌具有较强拮抗活性的菌株Blz67和Blz02。依据形态学观察及生理生化测试结果，并结合16S rRNA和gyrA基因序列分析，将两株拮抗菌鉴定为贝莱斯芽胞杆菌Bacillus velezensis。生物学特性测试结果表明:两株拮抗菌的最适生长温度均为31℃，最适生长pH均为5.5。盆栽试验结果表明，菌株Blz02与Blz67对香蕉枯萎病的防效分别为63.33%和40.00%，在香蕉枯萎病的生物防治上具有较好的应用前景。

关键词: 香蕉枯萎病, 贝莱斯芽胞杆菌, 生物防治

Abstract: Banana Fusarium wilt caused by Fusarium oxysporum f. sp. cubense (Foc) is a destructive disease in global banana industry. Till now, there is still no means available for effective control of the disease in field. In order to explore the biocontrol agents against banana Fusarium wilt, two antagonistic bacterial strains Blz67 and Blz02 were screened out by primary and secondary selections. The two strains were both identified as Bacillus velezensis by morphological observations, physiological and biochemical tests, combined with 16S rRNA and gyrA gene sequences analysis. The results of biological characteristics tests showed that the optimum temperature for growth of both strains were 31℃, and the optimum pH value both were 5.5. The control efficacies of the strains Blz02 and Blz67 against banana Fusarium wilt in green house were 63.33% and 40.00% under greenhouse conditions, respectively, indicating that both strains had a promising prospect in biological control of banana Fusarium wilt.

Key words: banana Fusarium wilt, Bacillus velezensis, biological control

中图分类号:

S476

杨迪, 杜婵娟, 张晋, 潘连富, 叶云峰, 黄思良, 付岗. 香蕉枯萎病拮抗菌贝莱斯芽胞杆菌的筛选鉴定及其生物学特性[J]. 中国生物防治学报, 2021, 37(1): 165-171.

YANG Di, DU Chanjuan, ZHANG Jin, PAN Lianfu, YE Yunfeng, HUANG Siliang, FU Gang. Screening, Identification and Biological Characteristics of Bacillus velezensis with Antagonst Activity against Banana Fusarium Wilt[J]. Chinese Journal of Biological Control, 2021, 37(1): 165-171.

参考文献

[1] 付岗, 叶云峰, 杜婵娟, 等. 香蕉枯萎病菌群体多样性研究进展[J]. 植物检疫, 2016, 30(2):7-12.
[2] 李华平, 李云锋, 聂燕芳. 香蕉枯萎病的发生及防控研究现状[J]. 华南农业大学学报, 2019, 40(5):128-136.
[3] 王振中. 香蕉枯萎病及其防治研究进展[J]. 中国植保导刊, 2006, 20(7):30-30.
[4] Bubici G, Kaushal M, Prigigallo M, et al. Biological control agents against Fusarium wilt of banana[J]. Frontiers in Microbiology, 2019, 10:616.
[5] 徐志周, 王明元, 杜锦鹏, 等. 一株香蕉枯萎病拮抗菌HQB-1的分离鉴定及其发酵条件优化[J]. 微生物学通报, 2019, 46(7):1611-1618.
[6] Sivamani E, Gnanamanickam S. Biological control of Fusarium oxysporum f. sp. cubense in banana by inoculation with Pseudomonas fluorescens[J]. Plant and Soil, 1988, 107:3-9.
[7] 殷晓敏, 陈弟, 吴红萍, 等. 一株对香蕉枯萎病菌具有良好拮抗作用的菊欧氏杆菌[J]. 中国生物防治, 2009, 25(1):60-65.
[8] 石妞妞, 杜宜新, 阮宏椿, 等. 枯草芽胞杆菌T122F内生定殖及对香蕉枯萎病的防治效果[J]. 植物保护, 2015, 41(4):95-99, 124.
[9] Wang B, Shen Z, Zhang F, et al. Bacillus amyloliquefaciens strain W19 can promote growth and yield and suppress Fusarium wilt in banana under greenhouse and field conditions[J]. Pedosphere, 2016, 26(5):733-744.
[10] Taribuka J, Wibowo A, Widyastuti S M, et al. Potency of six isolates of biocontrol agents endophytic Trichoderma against Fusarium wilt on banana[J]. Degraded and Mining Lands Management, 2017, 4(2):723-731.
[11] 农倩, 张雯龙, 蓝桃菊, 等. 一株抗香蕉枯萎病DSE菌株的筛选鉴定及抗病机理初探[J]. 热带作物学报, 2017, 38(3):559-564.
[12] 方中达. 植病研究方法(第三版)[M]. 北京:中国农业出版社, 1998.
[13] 付岗, 林善海, 黄思良, 等. 广西香蕉主要叶斑病菌拮抗微生物的分离筛选[J]. 西南农业学报, 2007, 20(3):421-424.
[14] 杜婵娟, 付岗, 潘连富, 等. 香蕉炭疽病拮抗菌Bs6602的诱变选育及防病作用研究[J]. 植物保护, 2016, 42(5):69-74,79.
[15] 东秀珠, 蔡妙英. 常见细菌系统鉴定手册[M]. 北京:科学出版社, 2001.
[16] Buchanan R E. 伯杰细菌鉴定手册(第八版)[M]. 北京:科学出版社, 1984.
[17] Gao Z, Zhang B, Liu H, et al. Identification of endophytic Bacillus velezensis ZSY-1 strain and antifungal activity of its volatile compounds against Alternaria solani and Botrytis cinerea[J]. Biological Control, 2017, 105:27-39
[18] 杜婵娟, 杨迪, 付岗, 等. 广西香蕉细菌性软腐病病原鉴定及生物学特性研究[J]. 植物保护, 2019, 45(5):148-156.
[19] 杜婵娟, 杨迪, 潘连富, 等. 香蕉枯萎病生防菌肥的优化及其防病促生效果研究[J]. 中国生物防治学报, 2020, 36(3):396-404.
[20] Ruiz García C, Béjar V, Martínez Checa F, et al. Bacillus velezensis sp. nov. a surfactant-producing bacterium isolated from the river Velez in Malaga, southern Spain[J]. International Journal of Systematic and Evolutionary Microbiology, 2005, 55(1):191-195.
[21] Borriss R, Chen X H, Rueckert C, et al. Relationship of Bacillus amyloliquefaciens clades associated with strains DSM 7(T) and FZB42(T):a proposal for Bacillus amyloliquefaciens sub sp. amyloliquefaciens subsp. nov. and Bacillus amyloliquefaciens sub sp. plantarum sub sp. nov. based on complete genome sequence comparisons[J]. International Journal of Systematic & Evolutionary Microbiology, 2011, 61(8):1786-1801.
[22] Dunlap C A, Kim S J, Kwon S W, et al. Bacillus velezensis is not a later heterotypic synonym of Bacillus amyloliquefaciens, Bacillus methylotrophicus, Bacillus amyloliquefaciens sub sp. plantarum and Bacillus oryzicola are later heterotypic synonyms of Bacillus velezensis based on phylogenomics[J]. International Journal of Systematic and Evolutionary Microbiology, 2016, 66:1212-1217.
[23] Fan B, Blom J, Klenk H P, et al. Bacillus amyloliquefaciens,Bacillus velezensis,and Bacillus siamensis form an "Operational Group B. amyloliquefaciens" within the B. subtilis species complex[J]. Frontiers in Microbiology, 2017, 8:22.
[24] Ye M, Tang X, Yang R, et al. Characteristic and application of a novel species of Bacillus:Bacillus velezensis[J]. Acs Chemical Biology, 2018, 10:1021.
[25] 陶永梅, 潘洪吉, 黄健, 等. 新型生防微生物因子贝莱斯芽孢杆菌(Bacillus velezensis)的研究与应用[J]. 中国植保导刊, 2019, 39(9):26-33.
[26] Nam M H, Kim H S, Lee H D, et al. Biological control of anthracnose crown rot in strawberry using Bacillus velezensis NSB-1[J]. Acta Horticulturae, 2014(1049):685-688.
[27] Meng Q, Jiang H, Hao J J. Effects of Bacillus velezensis strain BAC03 in promoting plant growth[J]. Biological Control, 2016, 98:18-26.
[28] 刘东岳, 李敏, 孙文献, 等. 拮抗尖孢镰刀菌的PGPR筛选与抑菌机制的初步研究[J]. 植物病理学报, 2017, 47(5):704-715.
[29] Cai X, Liu C, Wang B, et al. Genomic and metabolic traits endow Bacillus velezensis CC09 with a potential biocontrol agent in control of wheat powdery mildew disease[J]. Microbiological Research, 2017, 196:89-94.
[30] 宗英, 赵月菊, 刘阳, 等. 一株贝莱斯芽胞杆菌抑制禾谷镰刀菌的研究[J]. 核农学报, 2018, 32(2):310-317.
[31] Cao Y, Pi H, Chandrangsu P, et al. Antagonism of two plant-growth promoting Bacillus velezensis isolates against Ralstonia solanacearum and Fusarium oxysporum[J]. Scientific Reports, 2018, 8(1):4360.
[32] 沙月霞, 隋书婷, 曾庆超, 等. 贝莱斯芽胞杆菌E69预防稻瘟病等多种真菌病害的潜力[J]. 中国农业科学, 2019, 52(11):1908-1917.
[33] 李望梅, 张立丹, 刘芳, 等. pH值对香蕉枯萎病菌4号生理小种生长的影响[J]. 微生物学通报, 2019, 46(12):3286-3294.

香蕉枯萎病拮抗菌贝莱斯芽胞杆菌的筛选鉴定及其生物学特性

Screening, Identification and Biological Characteristics of Bacillus velezensis with Antagonst Activity against Banana Fusarium Wilt

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

联系我们

[1]	农向群, 闫多子, 蔡霓, 王广君, 涂雄兵, 张泽华. 真菌防治蝗虫研究进展[J]. 中国生物防治学报, 2021, 37(1): 11-29.
[2]	暴可心, 罗立平, 王小艺, 张彦龙, 魏可, 张永安, 杨忠岐. 害虫生物防治综合效益评价方法[J]. 中国生物防治学报, 2021, 37(1): 38-51.
[3]	杜霞, 刘霞, 周文武, 杨艳丽, 胡先奇, 高玉林. 马铃薯块茎蛾生物防治研究进展与展望[J]. 中国生物防治学报, 2021, 37(1): 60-69.
[4]	刘静雅, 李卓苗, 孟玲, 李保平. 捕食外来入侵扶桑绵粉蚧对异色瓢虫生活史特征的影响[J]. 中国生物防治学报, 2021, 37(1): 86-90.
[5]	徐文静, 隋丽, 高鹏, 张荣宝, 王曌, 张正坤, 李启云. 球孢白僵菌可湿性粉剂防治玉米螟的研究与应用[J]. 中国生物防治学报, 2020, 36(6): 862-865.
[6]	占军平, 张安明, 邓方坤, 卢森, 孙修炼. 甘蓝夜蛾核型多角体病毒悬浮剂防治草地贪夜蛾的应用与推广[J]. 中国生物防治学报, 2020, 36(6): 872-873.
[7]	武凤霞, 刘婧怡, 李吉进, 丁建莉, 张淑彬, 魏丹, 刘建斌. 堆肥茶生防作用影响因素及应用前景探讨[J]. 中国生物防治学报, 2020, 36(6): 972-980.
[8]	刘梅, 张昌容, 尚小丽, 郭军, 曾广, 石乐娟. 南方小花蝽对非洲菊上西花蓟马控制效果评价[J]. 中国生物防治学报, 2020, 36(6): 992-996.
[9]	阿尔孜姑丽·肉孜, 吐尔逊·阿合买提, 付开赟, 丁新华, 阿地力·沙塔尔, 郭文超. 新疆玉米种植区瓢虫资源调查及多样性分析[J]. 中国生物防治学报, 2020, 36(5): 697-707.
[10]	孙元星, 郝亚楠, 李明凌. 贮藏前补充人工饲料对七星瓢虫低温耐受力的影响[J]. 中国生物防治学报, 2020, 36(5): 708-713.
[11]	许帅, 谢学文, 张昀, 石延霞, 柴阿丽, 李磊, 李宝聚. 马铃薯枯萎病生防芽胞杆菌筛选及生防效果研究[J]. 中国生物防治学报, 2020, 36(5): 761-770.
[12]	何伟, 罗文芳, 许建军, 孙晓军. 贝莱斯芽胞杆菌JTB8-2的筛选、鉴定及其对瓜列当拮抗作用研究[J]. 中国生物防治学报, 2020, 36(5): 786-794.
[13]	郭义, 赵灿, 李君摘, 李敦松. 蠋蝽对荔枝蝽一龄若虫的捕食功能反应[J]. 中国生物防治学报, 2020, 36(5): 826-831.
[14]	田俊策, 鲁艳辉, 王国荣, 郑许松, 杨亚军, 徐红星, 方琦, 叶恭银, 臧连生, 吕仲贤. 种赤眼蜂对草地贪夜蛾卵的寄生能力研究[J]. 中国生物防治学报, 2020, 36(4): 485-490.
[15]	路子云, 杨小凡, 马爱红, 冉红凡, 刘文旭, 李建成. 管侧沟茧蜂对不同日龄草地贪夜蛾幼虫的寄生效果[J]. 中国生物防治学报, 2020, 36(4): 491-495.