耐辐射芽胞杆菌JK23的鉴定及其生防活性研究

doi:10.16409/j.cnki.2095-039x.2021.06.011

中国生物防治学报 ›› 2021, Vol. 37 ›› Issue (5): 997-1006.DOI: 10.16409/j.cnki.2095-039x.2021.06.011

耐辐射芽胞杆菌JK23的鉴定及其生防活性研究

张丽娟¹, 黄伟¹, 王宁¹, 宋博², 朱静¹, 热依罕姑丽·阿卜杜克依穆¹, 布坚乃提·阿巴斯¹, 王玮¹

1. 新疆农业科学院微生物应用研究所/新疆特殊环境微生物实验室, 乌鲁木齐 830091;
2. 农业部西北荒漠绿洲作物有害生物综合治理重点实验室/特色林果产业国家地方联合工程研究中心/新疆农业科学院植物保护研究所, 乌鲁木齐 830091

收稿日期:2021-02-23 出版日期:2021-10-08 发布日期:2021-10-28
通讯作者: 王玮,博士,研究员,E-mail:673662780@qq.com。
作者简介:张丽娟,硕士,副研究员,E-mail:zhanglijuan@xaas.ac.cn
基金资助:
新疆农业科学院农业科技创新平台能力提升建设专项（nkypt005）；新疆农业科学院青年科技骨干创新能力培养项目（xjnkq-2019019）；新疆维吾尔自治区重点实验室开放课题（2020D04032）

Identification of Radiation-resistant Bacillus velezensis JK23 and Its Biocontrol Activity

ZHANG Lijuan¹, HUANG Wei¹, WANG Ning¹, SONG Bo², ZHU Jing¹, ABDUKEYIM Rayhangul¹, ABBAS Bujannat¹, WANG Wei¹

1. Institute of Applied Microbiology, Xinjiang Academy of Agricultural Sciences/Xinjiang Laboratory of Special Environmental Microbiology, Urumqi 830091, China;
2. Key Laboratory of Integrated Pest Management on Crops in Northwestern Oasis Ministry of Agriculture/National Local Joint Engineering Research Center of Special Forestry and Fruit Industry/Institute of Plant Protection, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China

Received:2021-02-23 Online:2021-10-08 Published:2021-10-28

摘要/Abstract

摘要： 菌株JK23为本实验室耐辐射微生物资源库保藏的1株耐辐射芽胞杆菌，通过系统的形态学、生理生化实验及分子生物学鉴定，菌株JK23为贝莱斯芽胞杆菌。平板对峙试验表明，菌株JK23对多种植物病原菌都具有明显的抑制作用，抑制率分别为香梨黑斑病菌Alternaria Alternaria 39.68%、西瓜枯萎病菌Fusarium oxysporum SCC-F5 37.5%、水稻恶苗病菌Fusarium moniliforme 50.0%、棉花枯萎病菌Fusarium oxysporum SCC-F11 53.85%、马铃薯早疫病菌Alternaria solani 73.3%、西瓜蔓枯病菌Didymella bryoniae 100%、核桃腐烂病菌Cytcospora sp.100%。显微镜观察发现，菌株JK23能抑制病原真菌菌丝生长，引起菌丝畸形、膨大、固缩。通过基因检测及MALDI-TOF MS（基质辅助激光解吸电离飞行时间质谱）结果分析发现，菌株JK23能产生多种脂肽类抗生素，其产物具有较强的溶血和排油特性。鉴于贝莱斯芽胞杆菌JK23产生的脂肽类活性物质对多种植物病原真菌具有较好的抑制效果，表明其具有较高的开发应用前景。

关键词: 贝莱斯芽胞杆菌, 脂肽, 抑菌活性

Abstract: Strain JK23 is a radiation-resistant Bacillus sp. stored in our radiation-resistant microbial resource bank. Through systematic morphological, physiological and biochemical experiments and molecular identification, strain JK23 was confirmed as Bacillus velezensis. Plate confrontation experiment showed that strain JK23 had significant inhibitory effect on a variety of plant pathogens, and the inhibitory rates were as follows:Alternaria alternaria (39.68%), Fusarium oxysporum SCC-F5 (37.5%), Fusarium moniliforme SCC-F11 (50.0%), Fusarium oxysporum (53.85%), Alternaria solani (73.3%), Didymella bryoniae (100%) and Cytcospora sp. (100%). Meanwhile, using microscopic observation, strain JK23 demonstrated inhibiting effect on the growth of mycelia of pathogenic fungi, causing mycelia malformation, enlargement and pynosis. Genetic analysis and MALDI-TOF MS further discovered that strain JK23 could synthesize a large number of lipopeptide antibiotics, which had strong hemolysis and oil discharge characteristics. In view of the lipopeptide activity and inhibitory effect of B. velesiensis JK23, it has broad prospect for development and application in future.

Key words: Bacillus velezensis, lipopeptide, antifungal activity

中图分类号:

S476

张丽娟, 黄伟, 王宁, 宋博, 朱静, 热依罕姑丽·阿卜杜克依穆, 布坚乃提·阿巴斯, 王玮. 耐辐射芽胞杆菌JK23的鉴定及其生防活性研究[J]. 中国生物防治学报, 2021, 37(5): 997-1006.

ZHANG Lijuan, HUANG Wei, WANG Ning, SONG Bo, ZHU Jing, ABDUKEYIM Rayhangul, ABBAS Bujannat, WANG Wei. Identification of Radiation-resistant Bacillus velezensis JK23 and Its Biocontrol Activity[J]. Chinese Journal of Biological Control, 2021, 37(5): 997-1006.

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耐辐射芽胞杆菌JK23的鉴定及其生防活性研究

Identification of Radiation-resistant Bacillus velezensis JK23 and Its Biocontrol Activity

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