代森锰锌对异色瓢虫雌虫生殖毒性初探

doi:10.16409/j.cnki.2095-039x.2023.11.018

摘要/Abstract

摘要： 在农林业生产中，化学农药的使用成为了生物防治实施的制约因素之一。为了促进化学防治和生物防治的协调应用，评估农药对天敌昆虫的影响是天敌昆虫推广应用中的重要环节。异色瓢虫Harmoniaaxyridis是我国农业生产中常用的捕食性天敌之一，为了确保异色瓢虫在应用中的安全性，保障其持续发挥控害作用，本文采用毒蚜饲喂法，以卵巢发育情况为指标，评价了常用杀菌剂代森锰锌对异色瓢虫雌成虫的生殖毒性。试验结果表明，不同发育阶段的异色瓢虫在摄入代森锰锌后均会出现雌成虫卵巢发育受抑制的现象。主要表现为卵巢管长度下降、卵巢发育指数降低、Vg1和Vg2相对表达量降低。并且摄入代森锰锌的时间越长，对卵巢发育的抑制作用也越明显。此外，通过解剖观察未发现代森锰锌对异色瓢虫卵巢具有致畸作用。因此，推测代森锰锌对异色瓢虫具有较弱的生殖抑制作用。本文研究结果将增强代森锰锌与捕食性天敌昆虫应用的兼容性，提高代森锰锌与天敌昆虫的使用效率。

关键词: 代森锰锌, 异色瓢虫, 卵巢发育, 安全性评价

Abstract: In agricultural and forestry production,chemical pesticides have become one of the constraints against biological control.To coordinate chemical control with biological control,it is important to evaluate the effects of pesticides on natural enemy insects.Harmonia axyridis is one of the widespread predators widely used in agricultural production in China.To ensure the safety and the continuous control effect of H.axyridis during its application,this paper evaluated the reproductive toxicity of mancozeb to H.axyridis female adults by aphid feeding method.The ovary development degree and the relative expression of Vgs were investigated in the female predators fed with mancozeb.The results showed that the ovarian development was inhibited in the H.axyridis female adults,irrespective of the developmental stages when mancozeb was ingested.The main symptoms included decreases in follicle length,ovarian development index and the relative expression of Vg1 and Vg2.Consuming mancozeb since the larvae stage was more detrimental to the ovarian development compared to consuming since the adult stage.However,mancozeb treatment resulted in no abnormal ovary.Therefore,mancozeb probably has a weak reproductive toxicity effect on H.axyridis.The results can help improve the compatibility of mancozeb and predator and the efficiency of application of mancozeb and predator.

Key words: mancozeb, Harmonia axyridis, ovarian development, safety assessment

中图分类号:

S476.2

朱莉, 陈鑫, 蒲占湑, 吕佳, 黄振东, 鹿连明, 周兴苗. 代森锰锌对异色瓢虫雌虫生殖毒性初探[J]. 中国生物防治学报, 2024, 40(2): 477-483.

ZHU Li, CHEN Xin, PU Zhanxu, Lü Jia, HUANG Zhendong, LU Lianming, ZHOU Xingmiao. The Reproductive Toxicity of Mancozeb to Ladybeetle Harmonia Axyridis Pallas[J]. Chinese Journal of Biological Control, 2024, 40(2): 477-483.

参考文献

[1] Crnogorac G, Schwack W. Residue analysis of dithiocarbamate fungicides[J]. Trends in Analytical Chemistry, 2009, 28(1):40-50.
[2] 沈亚明,冯建国,袁小勇,等.保护性杀菌剂代森锰锌的应用研究概况[J].现代农药, 2017, 16(6):1-5.
[3] 朱鲁生.乙撑硫脲环境毒理研究进展[J].环境科学进展, 1995, 3(4):64-71.
[4] Arcanjo M E. Monitoring of mancozeb pesticide on the ground in different systems of tomato seeding[D]. Universidade do Estado do Rio de Janeiro, 2007.
[5] Seiler J P. Ethylenethiourea (ETU), a carcinogenic and mutagenic metabolite of ethylenebis-dithiocarbamate[J]. Mutation Research, 1974, 26(3):189-191.
[6] Teramoto S, Shingu A, Kaneda M, et al. Teratogenicity studies with ethylenethiourea in rats, mice and hamsters[J]. The Japanese Teratology Society, 1978, 18:11-17.
[7] Khera K S, Iverson F. Toxicity of ethylenethiourea in pregnant cats[J]. Teratology, 1978, 18(3):311-313.
[8] Vieira R, Venancio C A S, Felix L M. Toxic effects of a mancozeb-containing commercial formulation at environmental relevant concentrations on zebrafish embryonic development[J]. Environmental Science and Pollution Research, 2020, 27(17):21174-21187.
[9] Leandro L P, Mello R S, Silva D G C, et al. Behavioral changes occur earlier than redox alterations in developing zebrafish exposed to Mancozeb[J]. Environmental Pollution, 2021, 268:115783.
[10] Saraiva M A, Avila E R, Silva G F, et al. Exposure of Drosophila melanogaster to mancozeb induces oxidative damage and modulates Nrf2 and HSP70/83[J]. Oxidative Medicine and Cellular Longevity, 2018, 2018:5456928.
[11] Adamski Z, Ziemnicki K. Side-effects of mancozeb on Spodoptera exigua(Hübn.) larvae[J]. Journal of Applied Entomology, 2004, 128(3):212-217.
[12] 陈天业,牟吉元,慕立义,等.草蛉对农药的敏感性[J].中国生物防治, 1996, 12(1):39-42.
[13] 唐良德,邱宝利,任顺祥.天敌昆虫抗药性研究进展[J].应用昆虫学报, 2014, 51(1):13-25.
[14] 李浩,杨星科,刘强. 6种常用杀菌剂对苹果黄蚜及其天敌异色瓢虫的影响[J].环境昆虫学报, 2010, 32(1):60-65.
[15] Gao X, Hu F, Zhang S, et al. Glyphosate exposure disturbs the bacterial endosymbiont community and reduces body weight of the predatory ladybird beetle Harmonia axyridis(Coleoptera:Coccinellidae)[J]. Science of the Total Environment, 2021, 790:147847.
[16] 郭志芯,蒋红云,张兰,等.四种杀虫剂对七星瓢虫和松毛虫赤眼蜂的负效应[J].中国生物防治学报, 2019, 35(4):542-547.
[17] 谢连城,田俊策,鲁艳辉,等.稻飞虱靶标杀虫剂亚致死剂量多代处理对两种赤眼蜂的影响[J].中国生物防治学报, 2021, 37(5):920-926.
[18] 王甦,张润志,张帆.异色瓢虫生物生态学研究进展[J].应用生态学报, 2007, 18(9):2117-2126.
[19] 王小艺,沈佐锐.异色瓢虫的应用研究概况[J].昆虫知识, 2002, 39(4):255-261.
[20] 雒珺瑜,崔金杰,王春义,等.棉田释放异色瓢虫对棉蚜自然种群的控制效果[J].中国棉花, 2014, 41(7):8-10.
[21] 李金萍,郭喜红,侯峥嵘,等.释放异色瓢虫对北京春茬西瓜瓜蚜的控害效果[J].中国植保导刊, 2017, 37(4):38-41.
[22] 李姝,王甦,赵静,等.释放异色瓢虫对北京温室甜椒和圆茄上桃蚜的控害效果[J].植物保护学报, 2014, 41(6):699-704.
[23] 葛立傲,倪玺超,刘小英,等.异色瓢虫在茄子上防治蚜虫的应用研究[J].上海蔬菜, 2020, 6:52-53.
[24] 孙梅梅,柴伟纲,谌江华,等.人工释放异色瓢虫对甘蓝蚜的控制效果[J].浙江农业科学, 2015, 56(9):1452-1456.
[25] Twardowski J P, Hurej M, Twardowska K. Effect of ingestion exposure of selected insecticides on Coccinella septempunctata and Harmonia axyridis(Coleoptera:Coccinellidae)[J]. Insects, 2021, 12:434.
[26] Sakurai H, Hirano T, Takeda S. Physiological distinction between aestivation and hibernation in the lady beetle, Coccinella septempunctata bruckii (Coleoptera:Coccinellidae)[J]. Applied Entomology&Zoology, 1986, 21(3):424-429.
[27] 高俏.保幼激素对异色瓢虫生殖滞育发生的调控作用及机制研究[D].湖北:华中农业大学, 2021.
[28] Livak K J, Schmittgen T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2-△△CT method[J]. Methods, 2001, 25(4):402-408.
[29] Han H, Han S, Qin Q, et al. Molecular identification and functional characterization of vitellogenin receptor from Harmonia axyridis(Coleoptera:Coccinellidae)[J]. Journal of Economic Entomology, 2022, 115(1):325-333.
[30] Wu Z, Yang L, He Q, et al. Regulatory mechanisms of vitellogenesis in insects[J]. Frontiers in Cell and Developmental Biology, 2020, 8:593613.
[31] 姜晓环,王恩东,康杨,等.几种常见杀虫剂及杀菌剂对异色瓢虫的影响[J].植物保护, 2015, 41(4):151-153.
[32] Michaud J P, Angela K G. Sub-lethal effects of a copper sulfate fungicide on development and reproduction in three coccinellid species[J]. Journal of Insect Science, 2003, 3(16):1-6.
[33] Marchal-Ségault D, Seugé J, Laugé G, et al. Effects of ten commercial fungicides on adult survival of Drosophila melanogaster[J]. Journal of Environmental Science and Health, Part B:Pesticides, Food Contaminants, and Agricultural Wastes, 1985, 20(4):407-424.
[34] Ioriatti C, Pasqualini E, Toniolli A. Field and laboratory tests on selectivity of four fungicides on Amblyseius andersoni (Chant)(Acarina, Phytoseiidae)[J]. Bulletin of the Institute of Entomology, Guido Grandi of the University of Bologna, 1993, 47:155-168.
[35] Araujo G S, Pinheiro C, Pestana J L T, et al. Toxicity of lead and mancozeb differs in two monophyletic Daphnia species[J]. Ecotoxicology and Environmental Safety, 2019, 178:230-238.
[36] Carniel L S C, Niemeyer J C, Filho L C I O, et al. The fungicide mancozeb affects soil invertebrates in two subtropical Brazilian soils[J]. Chemosphere, 2019, 232:180-185.
[37] Mahadevaswami M P, Jadaramkunti U C, Hiremath M B, et al. Effect of mancozeb on ovarian compensatory hypertrophy and biochemical constituents in hemicastrated albino rat[J]. Reproductive Toxicology, 2000, 14(2):127-134.
[38] Rossi G, Buccione R, Baldassarre M, et al. Mancozeb exposure in vivo impairs mouse oocyte fertilizability[J]. Reproductive Toxicology, 2006, 21(2):216-219.
[39] Michaud J P. Responses of two ladybeetles to eight fungicides used in Florida citrus:Implications for biological control[J]. Journal of Insect Science, 2001, 1(1):6.