防御假单胞ZF509可湿性粉剂的研制及对瓜类细菌性果斑病的防治效果

doi:10.16409/j.cnki.2095-039x.2026.02.021

中国生物防治学报 ›› 2026, Vol. 42 ›› Issue (3): 660-671.DOI: 10.16409/j.cnki.2095-039x.2026.02.021

• 研究论文 • 上一篇

防御假单胞ZF509可湿性粉剂的研制及对瓜类细菌性果斑病的防治效果

赵子璇¹, 刘阳^1,2, 石延霞¹, 谢学文¹, 柴阿丽¹, 范腾飞¹, 孙先花¹, 李宝聚¹, 李磊¹

1. 中国农业科学院蔬菜花卉研究所/蔬菜生物育种全国重点实验室, 北京 100081;
2. 甘肃农业大学, 兰州 730000

收稿日期:2025-08-13 发布日期:2026-06-25
通讯作者: 李宝聚，博士，研究员，E-mail：libaojuivf@163.com;李磊，博士，研究员，E-mail：lilei01@caas.cn
作者简介:赵子璇，硕士研究生，E-mail：zyc2323016@163.com;刘阳，博士研究生，E-mail：liuy2723@163.com
基金资助:
国家重点研发计划(2024YFD2300700); 中国农业科学院科技创新工程(CAAS-ASTIP-IVFCAAS); 农业农村部园艺作物生物学与种质创制重点实验室(IVF2024); 蔬菜生物育种全国重点实验室开放课题

Development of Pseudomonas protegens ZF509-Based Wettable Powder Formulation and Its Efficacy in Controlling Bacterial blotch of fruit in Melons

ZHAO Zixuan¹, LIU Yang^1,2, SHI Yanxia¹, XIE Xuewen¹, CHAI Ali¹, FAN Tengfei¹, SUN Xianhua¹, LI Baoju¹, LI Lei¹

1. State Key Laboratory of Vegetable Biobreeding/Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China;
2. Gansu Agricultural University, Lanzhou 730000, China

Received:2025-08-13 Published:2026-06-25

摘要/Abstract

摘要： 为获得防治瓜类细菌性果斑病的高效生防菌剂，本研究基于生物相容性和质量指标，筛选获得防御假单胞菌ZF509相容性良好的可湿性粉剂配方，并测定其对瓜类细菌性果斑病盆栽防效试验。结果表明，可湿性粉剂最佳配方为90.5% ZF509发酵液，5%白炭黑载体，1% Morwet IP润湿剂，1%木质素苯磺酸钠分散剂，以及5%小麦淀粉保护剂；所得制剂活菌数为3.8×10⁹ cfu/g，质量指标符合国家标准。通过添加食品级小麦淀粉保护剂，提升了活性成分耐热性，37℃条件下ZF509存活率在80%以上。盆栽试验结果表明，防御假单胞菌ZF509可湿性粉剂1000倍液处理能有效防治瓜类细菌性果斑病，防治效果为49.52%，优于对照药剂噻唑锌。本研究为防御假单胞菌ZF509可湿性粉剂的研发与应用奠定了技术基础。

关键词: 防御假单胞菌, 瓜类细菌性果斑病, 可湿性粉剂, 防治效果

Abstract: To develop a highly effective biocontrol agent against bacterial fruit blotch(BFB), this study screened a wettable powder formulation with optimal biocompatibility and quality parameters for Pseudomonas protegens ZF509 and evaluated its protective efficacy in potted plants. The optimized formulation comprised: 90.5% ZF509 fermentation broth, 5% precipitated silica carrier, 1% Morwet IP wetting agent, 1% sodium lignosulfonate dispersant, and 5% food-grade wheat starch protectant. The resulting formulation achieved a viable cell count of 3.8×10⁹ cfu/g, and the quality indicators met the national standards. By adding food-grade wheat starch protectant, the heat resistance of the active ingredient was improved, and the survival rate of ZF509 was above 80% at 37 ℃. The pot experiment showed that the 1000-fold dilution of P. protegens ZF509 wettable powder could effectively control bacterial fruit blotch on melons, with a control efficacy of 49.52%, which was better than the control agent thiazole zinc. This study laid a technical foundation for the research and development and application of P. protegens ZF509 wettable powder.

Key words: Pseudomonas protegens, bacterial fruit blotch, wettable powder, control effect

中图分类号:

S476

赵子璇, 刘阳, 石延霞, 谢学文, 柴阿丽, 范腾飞, 孙先花, 李宝聚, 李磊. 防御假单胞ZF509可湿性粉剂的研制及对瓜类细菌性果斑病的防治效果[J]. 中国生物防治学报, 2026, 42(3): 660-671.

ZHAO Zixuan, LIU Yang, SHI Yanxia, XIE Xuewen, CHAI Ali, FAN Tengfei, SUN Xianhua, LI Baoju, LI Lei. Development of Pseudomonas protegens ZF509-Based Wettable Powder Formulation and Its Efficacy in Controlling Bacterial blotch of fruit in Melons[J]. Chinese Journal of Biological Control, 2026, 42(3): 660-671.

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防御假单胞ZF509可湿性粉剂的研制及对瓜类细菌性果斑病的防治效果

Development of Pseudomonas protegens ZF509-Based Wettable Powder Formulation and Its Efficacy in Controlling Bacterial blotch of fruit in Melons

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