牛角花齿蓟马对4种牧草花及其挥发物的行为选择反应

doi:10.16409/j.cnki.2095-039x.2023.09.002

摘要/Abstract

摘要： 为探明牛角花齿蓟马Odontothrips loti对寄主植物花及其挥发物的嗅觉行为反应,选取紫花苜蓿Medicago sativa、黄花草木樨Melilotus officinalis、红豆草Onobrychis viciaefolia和红三叶Trifolium pratense 4种豆科牧草,采用Y型嗅觉仪测定了蓟马对上述4种寄主植物的行为选择趋性,并采用动态顶空吸附法采集植物挥发物,气相色谱-质谱联用仪(GC-MS)鉴定化合物组分及含量分析。结果表明,与空气对照比较,牛角花齿蓟马对紫花苜蓿表现显著偏好选择,选择反应率为70.6%,对其他3种寄主的行为反应差异不显著。两项选择中,与红豆草、红三叶比较,牛角花齿蓟马显著趋向选择紫花苜蓿,选择反应率分别为78.3%和68.7%,其余各组合处理间均无显著差异。花释放的挥发物中,4种牧草共鉴定出40种化合物,分别是烷烃1种,醇类9种,酮类2种,醛类5种,酯类9种,萜烯类13种,酚类1种。因子分析显示,用主成分分析法提取的因子1, 2, 3分别解释了总方差概率的56.65%,24.47%和15.63%,对3个因子挥发物组分的贡献大小依次是紫花苜蓿、黄花草木樨、红豆草和红三叶。由此推测部分关键寄主挥发物苯甲醛、β-石竹烯、α-蒎烯、月桂烯、棕榈酸甲酯和百里酚等在牛角花齿蓟马与寄主化学通讯过程中发挥重要作用。研究结果为进一步开发植物源引诱剂进行牛角花齿蓟马的绿色防控提供了依据。

关键词: 牛角花齿蓟马, 紫花苜蓿, 挥发物, 嗅觉

Abstract: The olfactory behavior responses of the thrip Odontothrips loti female adults to their host plants, four leguminous forages, Medicago sativa, Melilotus officinalis, Onobrychis viciaefolia, and Trifolium pratense, were tested using 'Y' type olfactometer. The dynamic headspace sampling was used to collect plant volatiles, and gas chromatography-mass spectrometry (GC-MS) was used to identify the chemical composition and content of the volatiles. The results showed that female adults of O. loti significantly preferred flower odors of M. sativa with a selection rate of 70.6%, while showed no significant preference for the other three hosts, M. officinalis, O. viciaefolia and T. pratense. In dual-choice behavioral assays, O. loti females were significantly attracted to M. sativa in comparison with O. viciaefolia and T. pratense, with a selection rate of 78.3% and 68.7%, respectively. There were no significant differences in preference in other treatment combinations. A total of 40 volatile components, including 1 alkane, 9 alcohols, 2 ketones, 5 aldehydes, 9 esters, 13 terpenes, and 1 phenol, were identified from the volatiles. The factors 1, 2 and 3 extracted by principal component analysis explained 56.65%, 24.47% and 15.63% of the total variance, respectively. The contribution to the three factors to volatile components ranked from high to low was M. sativa, M. officinalis, O. viciaefolia, and T. pratense. The crucial host plant volatile components, such as benzaldehyde, β-Caryophyllene, (+)-α-pinene, myrcene, methyl palmitate, and thymol, probably play important roles in chemosensory communication between O. loti and its host plants. These results provide a basis for further development of plant-derived attractants for the green control of O. loti.

Key words: Odontothrips loti, Medicago sativa, volatile, olfactory

中图分类号:

S541

崔晓宁, 赵晓东, 席驳鑫, 胡桂馨, 尚素琴, 彭斌, 史薛强. 牛角花齿蓟马对4种牧草花及其挥发物的行为选择反应[J]. 中国生物防治学报, 2023, 39(4): 824-833.

CUI Xiaoning, ZHAO Xiaodong, XI Boxin, HU Guixin, SHANG Suqin, PENG Bin, SHI Xueqiang. Olfactory Behavior Responses of Odontothrips loti Female Adults to Four Leguminous Plants and their Volatiles[J]. Chinese Journal of Biological Control, 2023, 39(4): 824-833.

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