Welcome to Chinese Journal of Biological Control,Today is

journal1

Previous Articles    

Identification of Bacillus thuringiensis from Opisina arenosella and Determination of Its Insecticidal Bioactivity

SUN Xiaodong1,2, YAN Wei1, LI Chaoxu1, LÜ Chaojun1, TAN Weiquan1   

  1. 1. Coconut Research Institute, Chinese Academy of Tropical Agricultural Sciences, Wenchang 571339, China;
    2. College of Science, Northeast Agricultural University, Harbin 150030, China
  • Received:2015-06-30 Online:2016-04-08 Published:2016-04-08

Abstract: A virulent strain WZS171 with high infectivity was isolated from naturally infected and dead larvae of Opisina arenosella Walker collected from Hainan Wuzhishan. The strain was evaluated for its insecticidal bioactivities. The strain was identified as Bacillus thuringiensis by morphological tests and analysis of cry genes. The new genes of cry1Aa13, cry1Gb1, cry1D, cry1Ia, cry2Af were found in this isolate. NCBI BLAST revealed that the nucleotide fragments of these genes showed homology of 98%, 99%, 90%, 92% and 99%, respectively, with the known genes at the GeneBank. Bioactivity of WZS171 against the larvae of Opisina arenosella Walker was assayed in laboratory. WZS171 showed high insecticidal activity at 72 h post-treatment, with a LC50 of 9.08 μg/mL and a confidence interval of 6.72-12.26 μg/mL at the 95% confidence level. These results show that WZS171 is host specific in virulence and highly virulent against larvae of Opisina arenosella Walker.

Key words: Bacillus thuringiensis, Opisina arenosella Walker, identification of cry gene, insecticidal activity