Study on the Efficiency of Functional Multi?microbial Agent against Root-knot Nematode Meloidogyne spp. on Cucumber

doi:10.16409/j.cnki.2095-039x.2016.04.011

Abstract

Abstract: Root-knot nematode (Meloidogyne spp.) is a kind of important soil-borne pathogen, which can cause serious economic losses in the production of vegetables worldwide. Biological control strategy is an environmental safe and effective method to control this destructive pathogen. In this study, we constructed a functional composite microbial community (Purpureocillium lilacinum+Streptomyces rubrogriseus+Ochrobactrum sp.) with good biological compatibility and complementary function. The effects of the multi-microbial agent on cucumber root-knot nematode and the plant biomass and yield were evaluated in the field. The results showed that nematode reproduction index (RI) was the lowest in the soil applied with the multi-microbial agent compared with other applications. The multi-microbial agent reduced gall index to 2.0 and 4.6, and the control efficiencies were 56.5% and 42.5% at 38 and 105 d, respectively, which were higher than those of 10% fosthiazate. Application of the multi-microbial agent could not only promote the plant shoot growth but also increased the yield of cucumber. Our study provides a new insight for the biological control of Meloidogyne spp..

Key words: Meloidogyne spp., biological control, synthetic microbial community, multi-microbial agent

CLC Number:

S476

JI Yu, WU Yunpeng, WANG Yin, WANG Xiaoqing, LIU Xingzhong, LIU Jianbin, XIANG Meichun. Study on the Efficiency of Functional Multi?microbial Agent against Root-knot Nematode Meloidogyne spp. on Cucumber[J]. journal1, 2016, 32(4): 493-502.

References

[1] Huang W K, Sun J H, Cui J K, et al. Efficacy Evaluation of Fungus Syncephalastrum racemosum and nematicide avermectin against the root-knot nematode Meloidogyne incognita on cucumber[J]. PLoS ONE, 2014, 9(2): 1-6.
[2] 姜力利, 刘霆, 刘伟成, 等. 里西裸囊菌za-130的杀线活性及其影响因素[J]. 中国生物防治学报, 2010, 26(4): 461-466.
[3] 王振, 李世东, 缪作清, 等. 有机物与淡紫紫孢霉对番茄根结线虫病的协同防治[J]. 中国生物防治学报, 2015, 31(1): 130-138.
[4] Khan Z, Kim S G, Jeon Y H, et al. A plant growth promoting rhizobacterium, Paenibacillus polymyxa strain GBR-1, suppresses root-knot nematode[J]. Bioresource Technology, 2008, 99(8): 3016-3023.
[5] Li J, Zou C, Xu J, et al. Molecular mechanisms of nematode-nematophagous microbe interactions: basis for biological control of plant-parasitic nematodes[J]. Annual Review of Phytopathology, 2015, 53: 67-95.
[6] Meyer S L F, Roberts D P. Combination of biocontrol agents for management of plant-parasitic nematodes and soil-borne plant-pathogenic fungi[J]. Journal of Nematology, 2002, 34(1): 1-8.
[7] Roberts D P, Lohrke S M, Meyer S L F, et al. Biocontrol agents applied individually and in combination for suppression of soilborne diseases of cucumber[J]. Crop Protection, 2005, 24(2): 141-155.
[8] van Elsas J D, Chiruazzi M, Mallon C A, et al. Microbial diversity determines the invasion of soil by a bacteria pathogen[J]. Proceeding of the National Academy of Sciences of the United States of America, 2012, 109(4): 1159-1164.
[9] Wei Z, Yang T, Friman V P, et al. Trophic network architecture of root-associated bacterial communities determines pathogen invasion and plant health[J]. Nature Communications, 2014, 6: 1-9.
[10] Srinivasan K, Mathivanan N. Biological control of sunflower necrosis virus disease with powder and liquid formulations of plant growth promoting microbial consortia under field conditions[J]. Biological Control, 2009, 51(3): 395-402.
[11] Maherali H, Klironomos J N. Influence of phylogeny on fungal community assembly and ecosystem functioning[J]. Science, 2007, 316(5832): 1746-1748.
[12] Sharma A, Sharma S, Dalela M. Nematicidal activity of Paecilomyces lilacinus 6029 culture on Karanja cake medium[J]. Microbial Pathogenesis, 2014, 75: 16-20.
[13] 张家家, 李世东, 郭荣君, 等. 淡紫紫孢菌YES和粉红螺旋聚孢霉67-1组合对黄瓜根结线虫病的防效评价[J]. 中国生物防治学报, 2014, 30(6): 787-794.
[14] 陈立杰, 陈井生, 郑雅楠, 等. 放线菌Snen253的鉴定及对大豆孢囊线虫的抑制作用[J]. 中国生物防治学报, 2009, 25(1): 66-69.
[15] Xu S, Liu Y, Wang J J, et al. Isolation and potential of Ochrobactrum sp. NW-3 to increase the growth of cucumber[J]. International Journal of Agricultural Policy and Research, 2015, 3(9): 341-350.
[16] Imran A, Saadalla M J A, Khan S U, et al. Ochrobactrum sp. Pv2Z2 exhibits multiple traits of plant growth promoting, biodegradation and N-acyl-homoserine-lactone quorum sensing[J]. Annals of Microbiology, 2014, 64(4): 1797-1806.
[17] Sun M H, Gao L, Shi Y X, et al. Fungi and actinomycetes associated with Meloidogyne spp. eggs and females in China and their biocontrol potential[J]. Journal of Invertebrate Pathology, 2006, 93(1): 22-28.
[18] 刘建斌. 基于微生物群落原理的防治根结线虫复合菌剂研究[D]. 北京: 中国科学院微生物研究所, 2013.
[19] 薛慧. 线虫生防菌—红灰链霉菌发酵工艺研究及生防作用的可行性评价[D]. 北京: 中国农业大学, 2011.
[20] Jenkins W R. A rapid centrifugal-flotation technique for separating nematodes from soil[J]. Plant Disease Reporter, 1964, 48: 692.
[21] Ruano-Rose D, Cazorla E M, Bonilla N. Biological control of avocado white root rot with combined applications of Trichoderma spp. and rhizobacteria[J]. Europe Journal of Plant Pathology, 2014, 138(4): 751-762.
[22] Wang L, Feng Y, Tian J Q, et al. Farming of a defensive fungal mutualist by an attelabid weevil[J]. The ISME Journal, 2015, 9(8): 1793-1801.
[23] Requena A M, Candela M E, Requena M E, et al. Host-pathogen interaction of root-knot nematode Meloidogyne incognita on pepper in the southeast of Spain[J]. Europe Journal of Plant Pathology, 2011, 131: 515-518.
[24] Bridge J, Page S L J. Estimation of root-knot nematode infestation levels on roots using a rating chart[J]. Tropical Pest management, 1980, 26(3): 296-298.
[25] Wei L, Shao Y, Wan J, et al. Isolation and characterization of a rhizobacterial antagonist of root-knot nematodes[J]. PLoS ONE, 2014, 9(1): e85988.
[26] Liu R J, Dai M, Wu X, et al. Suppression of the root-knot nematode Meloidogyne incognita on tomato by dual inoculation with arbuscular mycorrhizal fungi and plant growth-promoting rhizobacteria[J]. Mycorrhiza, 2012, 22(4): 289-296.
[27] Huang W K, Cui J K, Liu S M, et al. Testing various biocontrol agents against the root-knot nematode (Meloidogyne incognita) in cucumber plants identifies a combination of Syncephalastrum racemosum and Paecilomyces lilatinus as being most effective[J]. Biological Control, 2016, 92: 31-37.
[28] 张峰, 李英梅, 洪波, 等. 温度和初始接种量对南方根结线虫侵染力的影响[J]. 中国生态农业学报, 2012, 20(12): 1631-1635.
[29] Xue C, Penton C R, Shen Z Z, et al. Manipulating the banana rhizosphere microbiome for biological control of panama disease[J]. Scientific Reports, 2015, 5: 1-13.
[30] Mariana S C, Gerard W K, Johnny H M, et al. Chitin amendment increases soil suppressiveness towards plant pathogens and modulates the actinobacterial and oxalobacteraceal communities in an experimental agricultural field[J]. Applied and Environmental Microbiology, 2013, 79(17): 5291-5301.