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Chinese Journal Of Biological Control ›› 2020, Vol. 36 ›› Issue (2): 189-195.DOI: 10.16409/j.cnki.2095-039x.2020.02.004

• RESEARCH REPORTS • Previous Articles     Next Articles

Morbidity of Chilo suppressalis in Wild and Cultivated Water Oat (Zizania latifolia) and Microorganism Diversity in Dead Insects

LIU Yue1, GUO Hao1, HUANG Xinfang2, ZHANG Xiaoli1, LI Duo1, WANG Yongmo1   

  1. 1. College of plant science & technology, Huazhong Agricultural University, Wuhan 430070, China;
    2. Institute of Vegetables, Wuhan Institute of Agricultural Sciences, Wuhan 430345, China
  • Received:2019-09-18 Online:2020-04-08 Published:2020-04-16

Abstract: Water oat Zizania latifolia is an aquatic vegetable in China, with high nutritional value and good taste. In recent years, the vegetable has been cultivated increasingly. However, water oat is susceptible to insect pests among which Chilo suppressalis is one of the most serious. Insect pathogens are effective biological control agents of pest insects. In order to find effective insect pathogens that prevail in water oat field, 6039 overwintering C. suppressalis larvae were collected from wild and cultivated water oat, and were observed for the mortality due to insect pathogens, which revealed no difference in pathogenic mortality of 19.98%in wild water oat and of 17.00% in cultivated water oat, indicating that farming practices do not affect the prevailing of insect pathogens. Microorganism community in the dead specimens (>1000) were identified by high-throughput sequencing of 18S rRNA and 16S rRNA gene. Results showed that 96.67% of dead specimens from wild water oat were predominated by Beauveria fungi, and 100% of dead specimens from cultivated water oat were predominated by this genus. Except for one sample, the abundance of Beauveria in each specimen was more than 90%. Metarhizium fungi were not detected in any of the specimens. Enterobacteriaceae, Rhizobiaceae, Enterococcaceae and Comamonadaceae were the top four bacteria family with abundances of 19.37%, 17.37%, 7.04% and 5.04%, respectively. Insect pathogenic bacteria were seldom reported in those four dominant families. Bacillaceae and Clostridiaceae that most likely contain insect pathogenic bacteria were only 1.44% and 0.41% in abundance. This study shows that Beauveria is the dominant pathogen of C. suppressalis during the overwintering stage, and is of potential as biocontrol agent against C. suppressalis in water oat field.

Key words: Zizania latifolia, Chilo suppressalis, biological control, insect pathogen, Beauveria

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