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journal1 ›› 2016, Vol. 32 ›› Issue (6): 783-787.DOI: 10.16409/j.cnki.2095-039x.2016.06.016

• RESEARCH REPORTS • Previous Articles     Next Articles

Identification of Antifungal Compound from Streptomyces rimosus M527 and Its Application in Biocontrol of Pathogenic Fusarium Wilt on Cucumber

LU Dandan1, ZHAO Yanfang1, MA Zheng1, ZHANG Yongyong1, WANG Jidong2, YU Xiaoping1   

  1. 1. Zhejiang Provincial Key Laboratory of Biometrology and Inspection and Quarantine/College of Life Sciences, China Jiliang University, Hangzhou 310018, China;
    2. Zhejiang Hisun Pharmaceutical Company Limited, Taizhou 318000, China
  • Received:2016-04-16 Online:2016-12-08 Published:2016-12-07

Abstract: Using Fusarium oxysporum f. sp. cucumerinum as an indicator strain, the antifungal active component from the fermentation broth of the strain Streptomyces rimosus M527 were identified and its antagonistic activity against fusarium wilt of cucumber were investigated. After the fermentation broth of S. rimosus M527 was extracted with organic solvent and separated on silica gel column chromatography, the active compound was further purified by chromatography on Sephadex LH-20 and preparative HPLC. Based on the structure analyses of the active compound by MS, 1H-NMR and 13C-NMR spectroscopy, the active compound was identified to be rimocidin. Rimocidin at the concentration of 2.20 mg/L could completely inhibit the spore germination of F. oxysporum f. sp. cucumerinum, while the hyphal growth could be inhibited by 8.78 mg/L rimocidin. Greenhouse experiments on potted cucumber plants demonstrated that therapeutic effect of rimocidin was better than that of ethylicin. After treatment for 14 d and 21 d, the therapeutic effect of rimocidin (17.56 mg/L) against F. oxysporum f. sp. cucumerinum in potted cucumber seeding was 78.3% and 70.6%, respectively, while the 1000 fold diluted ethylicin (80%) was 66.7% and 42.7%, respectively.

Key words: purification and identification, rimocidin, Fusarium oxysporum f. sp. cucumerinum, biological control

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