Biological Control of Crown Gall Disease using Agrobacterium rhizogenes Strain K1026

doi:10.16409/j.cnki.2095-039x.2017.03.017

Abstract

Abstract: Crown gall, cause by pathogenic Agrobacterium spp., is a serious problem in the production of cherry, peach and other fruits in Shandong and other provinces of China. The biological control agent Agrobacterium rhizogenes K1026 was developed in Australia and has been used commercially in a number of countries. However, K1026 has not been registered or made commercially available in China. Pathogenic agrobacteria were isolated from galls of cherry and peach, and soil collected from a cherry nursery in Shandong Province. More than 100 isolates were classified as Agrobacterium rhizogenes. The sensitivities of 37 isolates to K1026 were tested on Stonier's medium. All isolates were sensitive to antibiotics produced by strain K1026, exhibiting at least five different types of inhibition pattern. Crown gall caused by all 37 pathogenic strains was completely controlled by inoculation with strain K1026 in a carrot root disc bioassay. This result suggests that biocontrol of crown gall on cherry and peach in Shandong using A. rhizogenes K1026 is feasible.

Key words: crown gall, Agrobacterium rhizogenes, biological control, cherry, peach

CLC Number:

S476

WEI Yanli, YANG Hetong, LI Jishun, HU Jindong, LI Guotian, RYDER Maarten. Biological Control of Crown Gall Disease using Agrobacterium rhizogenes Strain K1026[J]. journal1, 2017, 33(2/3): 415-420.

References

[1] Li S, Zhang F, Sun Q. The reviewed of cherry tree crown gall disease[J]. Yantai Fruits, 2010, 110: 7-9.
[2] Jones D A, Ryder M H, Clare B G, et al. Construction of a Tra-deletion mutant of pAgK84 to safeguard the biological control of crown gall[J]. Molecular and General Genetics, 1988, 212: 207-214.
[3] Jones D A, Kerr A. Agrobacterium radiobacter K1026, a genetically-engineered derivative of strain K84, for biological control of crown gall[J]. Plant Disease, 1989, 73: 15-18.
[4] Kerr A. Biological control of crown gall[J]. Australasian Plant Pathology, 2016, 45(1): 15-18.
[5] Wang H M, Liang Y J, Wang J H, et al. Study on the crown gall disease control of stone fruit trees with anti-gall agent[J]. Plant Protection[China], 1995, 21: 24-26.
[6] Wang H Y, Wang H M, Wang J H, et al. The pathogen of crown gall disease on flowering cherry and its sensitivity to strain K1026[J]. European Journal of Plant Pathology, 2000, 106: 475-479.
[7] Brisbane P G, Kerr A. Selective media for the three biovars of Agrobacterium[J]. Journal of Applied Bacteriology, 1983, 54: 425-431.
[8] Ryder M H, Tate M E, Kerr A. Virulence properties of strains of Agrobacterium on the apical and basal surfaces of carrot root discs[J]. Plant Physiology, 1985, 77: 215-221.
[9] Stonier T. Agrobacterium tumefaciens Conn. II. Production of an antibiotic substance[J]. Journal of Bacteriology, 1960, 79: 889-898.
[10] Donner S C, Jones D A, McClure N C, et al. Agrocin 434, a new plasmid encoded agrocin from the biocontrol Agrobacterium strains K84 and K1026, which inhibits biovar 2 agrobacteria[J]. Physiological and Molecular Plant Pathology, 1993, 42(3): 185-194.
[11] Ellis J G, Murphy P J. Four new opines from crown gall tumours-their detection and properties[J]. Molecular and General Genetics, 1981, 181: 36-43.
[12] McClure N C, Ahmadi A R, Clare B G. Construction of a range of derivatives of the biological control strain Agrobacterium rhizogenes K84: a study of factors involved in biological control of crown gall disease[J]. Applied and Environmental Microbiology, 1998, 64: 3977-3982.
[13] Penyalver R, Oger P, Lopez M M, et al. Iron-binding compounds from Agrobacterium spp.: biological control strain Agrobacterium rhizogenes K84 produces a hydroxamate siderophores[J]. Applied and Environmental Microbiology, 2001, 67: 654-664.
[14] Lippincott J A, Lippincott B B. The genus Agrobacterium and plant tumorigenesis[J]. Annual Review of Microbiology, 1975, 29: 377-405.

[1]	AERZIGULI Rouzi, TURSUN·Ahmat, FU Kaiyun, DING Xinhua, ADILI Shataer, GUO Wenchao. Investigation and Diversity Analysis of Lady Beetle Resources in Corn Producing Areas of Xinjiang [J]. Chinese Journal Of Biological Control, 2020, 36(5): 697-707.
[2]	SUN Yuanxing, HAO Yanan, LI Mingling. Effect of Supplementation of Artificial Diet before Storage on Cold Tolerance of Coccinella septempunctata [J]. Chinese Journal Of Biological Control, 2020, 36(5): 708-713.
[3]	XU Shuai, XIE Xuewen, ZHANG Yun, SHI Yanxia, CHAI Ali, LI Lei, LI Baoju. Screening of Biocontrol Bacillus Isolate against Potato Fusarium Wilt and Its Biocontrol Effect [J]. Chinese Journal Of Biological Control, 2020, 36(5): 761-770.
[4]	HUANG Jiexue, WANG Xiaolin, WU Jie, JI Muxiang. Application of Microbial Biocontrol Agents Combined with Organic Material Addition for Combating Strawberry Gray Mold [J]. Chinese Journal Of Biological Control, 2020, 36(5): 802-810.
[5]	TIAN Junce, LU Yanhui, WANG Guorong, ZHENG Xusong, YANG Yajun, XU Hongxing, FANG Qi, YE Gongyin, ZANG Liansheng, Lü Zhongxian. The Parasitic Capability of Five Trichogramma Species on Eggs of Fall Armyworm Spodoptera frugiperda [J]. Chinese Journal Of Biological Control, 2020, 36(4): 485-490.
[6]	LU Ziyun, YANG Xiaofan, MA Aihong, RAN Hongfan, LIU Wenxu, LI Jiancheng. Parasitic Potential of Microplitis tuberculifer on Spodoptera frugiperda Larvae [J]. Chinese Journal Of Biological Control, 2020, 36(4): 491-495.
[7]	HUANG Chaolong, TANG Yin, HE Kanglai, WANG Zhenying. Predation of Chlaenius bioculatus Larvae to Larvae of Spodoptera frugiperda (Lepidoptera: Noctuidae) [J]. Chinese Journal Of Biological Control, 2020, 36(4): 507-512.
[8]	LI Ping, LI Yuyan, XIANG Mei, WANG Mengqing, MAO Jianjun, CHEN Hongyin, ZHANG Lisheng. Predation Capacity of Chrysopa pallens Larvae to Young Larvae of Spodoptera frugiperda [J]. Chinese Journal Of Biological Control, 2020, 36(4): 513-519.
[9]	WANG Yanan, ZHAO Shengyuan, HE Yunzhuan, WU Kongming, LI Guoping, FENG Hongqiang. Predation of the Larvae of Spodoptera frugiperda (J. E. Smith) by Sycanus croceouittatus Dohrn [J]. Chinese Journal Of Biological Control, 2020, 36(4): 525-529.
[10]	PAN Hongsheng, LI Haobin, DING Ruifeng, LI Haiqiang, WANG Dongmei, AKEDAN·Wuwaishi, LIU Jian. Predation Capacity of Adonia variegata to Aphis atrata [J]. Chinese Journal Of Biological Control, 2020, 36(4): 628-631.
[11]	LIAO Ping, SHI Xinru, GUO Yi, YIN Yanfang, ZHU Yanjuan, LI Yuyan, MAO Jianjun, WANG Mengqing, ZHANG Lisheng, CHEN Hongyin, LIU Chenxi. Influence of Low Temperature on Growth and Development of Arma chinensis Fallou (Hemiptera: Pentatomidae) [J]. Chinese Journal Of Biological Control, 2020, 36(3): 340-346.
[12]	LI Lei, ZHAO Yurong, ZHENG Fei, SHI Yanxia, CHAI Ali, XIE Xuewen, LI Baoju. Screening and Biocontrol of Antagonistic Bacillus against Celery Soft Rot [J]. Chinese Journal Of Biological Control, 2020, 36(3): 388-395.
[13]	JIA Ruimin, HU Lifang, WANG Tongtong, MA Qing, WANG Yang. Studies on Control Efficacy and Growth-Promotion Effect of Three Antagonistic Microbial Strains on Clubroot of Brassica oleracea [J]. Chinese Journal Of Biological Control, 2020, 36(3): 405-413.
[14]	LIU Yue, GUO Hao, HUANG Xinfang, ZHANG Xiaoli, LI Duo, WANG Yongmo. Morbidity of Chilo suppressalis in Wild and Cultivated Water Oat (Zizania latifolia) and Microorganism Diversity in Dead Insects [J]. Chinese Journal Of Biological Control, 2020, 36(2): 189-195.
[15]	LU Xiuyun, ZHAO Weisong, BAI Ying, MA Li, ZHANG Lihong, SHANG Junyan, SU Zhenhe, GUO Qinggang, ZHANG Xiaoyun, WANG Peipei, YAN Lei, MA Ping, LI Shezeng. Biological Control of Potato Verticillium Wilt by Microbial Fungicide [J]. Chinese Journal Of Biological Control, 2020, 36(2): 280-287.