Synergism between Crude Ethanol Extract of Eclipta prostrata with Cry1Ca and Its Influence on the Expression of ATP-binding Cassette Transporters C mRNA in Spodoptera exigua

doi:10.16409/j.cnki.2095-039x.2017.04.003

Abstract

Abstract: The objective of this study was to determine the synergistic effect of crude ethanol extract of Eclipta prostrata (CEEEP) on toxicity of Cry1Ca against Spodoptera exigua and the changes of expression of SeABCC1, SeABCC2 and SeABCC3. The results showed that CEEEP had an obvious poisoning effect on the 2nd instar larvae of S. exigua. When fed 5.26 mg/cm² of CEEEP for 7 days, the mortality rate was 58.4%. When S. exigua ingested the admixture of LD₃₀ of CEEEP and Cry1Ca, larval mortalities were 59.72%, 73.61% and 83.33% after 3 d, 5 d and 7 d. However, with LD₃₀ of Cry1Ca alone, the mortalities were 5.6%, 13.9%, 31.9%, respectively, and with CEEEP alone, the mortalities were 21.8%, 28.7%, 30.1%, respectively. The relative expression levels of SeABCC1, SeABCC2 and SeABCC3 were analyzed by qPCR, CEEEP can induce high expression of SeABCC1, SeABCC2 and SeABCC3, causing the larvae of S. exigua more susceptibility to Cry1Ca. In summary, CEEEP as botanical pesticide can be mixed with Bt toxin, to improve the control effect against S. exigua.

Key words: crude ethanol extract of Eclipta prostrata, Cry1Ca, Spodoptera exigua, toxicity, ATP-binding cassette transporters C, synergism

CLC Number:

S476.1

WANG Yingying, REN Xiangliang, JIANG Weili, HU Hongyan, MA Yajie, LI Qian, MA Yan. Synergism between Crude Ethanol Extract of Eclipta prostrata with Cry1Ca and Its Influence on the Expression of ATP-binding Cassette Transporters C mRNA in Spodoptera exigua[J]. journal1, 2017, 33(4): 446-453.

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