Anti-oomycete Activity of Cinnamon Essential Oil against Phytophthora capsici

doi:10.16409/j.cnki.2095-039x.2018.03.003

Abstract

Abstract: The aim of this study was to evaluate the effectiveness of cinnamon essential oil on controlling pepper blight caused by Phytophthora capsici. The toxicities of the essential oil against P. capsici at their four different life stages were examined in vitro conditions and the control efficacies were also investigated in vivo conditions. Moreover, morphology and structure of mycelial treated with the essential oil were observed and the effect of the essential oil on the permeability of the cell membrane was determined. The results showed that the EC₅₀ value of cinnamon essential oil against the mycelial growth was 111.89 mg/L. The oil could effectively inhibit sporangium formation, sporangium germination and zoospore germination with EC₅₀ values of 61.09 mg/L, 95.14 mg/L and 54.88 mg/L, respectively. The zoospore of the tested pathogen strain was sensitive to the cinnamon essential oil. The mycelial fresh weight and dry weight were significantly decreased by the essential oil. Morphological and structural observation showed that cinnamon essential oil caused the excessive branching and shortening of hyphae. The conductivity of mycelia cell suspension increased in the presence of the essential oil, indicating that the oil affected the cell membrane permeability. The conductivities of mycelia treated by essential oil at four concentrations increased with time, significantly higher than that of control. The above results showed that cinnamon essential oil had good protective and therapeutic effects on pepper blight, and the protective effect was stronger than the therapeutic effect. Therefore, cinnamon essential oil is worthy of further study and has a strong application potential.

Key words: cinnamon essential oil, Phytophthora capsici, life stage, toxicity, control efficacy

CLC Number:

S476

WANG Haijiao, WANG Yinan, GAO Fei, DUAN Pengfei, LIU Xiangyang, YOU Xiufeng, YAO Lunguang, ZHAO Te, ZHOU Lin. Anti-oomycete Activity of Cinnamon Essential Oil against Phytophthora capsici[J]. journal1, 2018, 34(3): 461-468.

References

[1] Kousik C S, Donahoo R S, Hassell R. Resistance in watermelon rootstocks to crown rot caused by Phytophthora capsici[J]. Crop Protection, 2012, 39(5):18-25.
[2] 李惠霞, 刘永刚, 漆永红. 12种杀菌剂对辣椒疫霉菌的室内毒力比较[J]. 甘肃农业大学学报, 2006, 41(5):63-66.
[3] Hausbeck M K, Lamour K H. Phytophthora capsici on vegetable crops:research progress and management challenges[J]. Plant Disease, 2004, 88(12):1292-1303.
[4] Sholberg P L, Walker M C, O'Gorman D T, et al. First report of Phytophthora capsici on cucurbits and peppers in British Columbia[J]. Canadian Journal of Plant Pathology, 2007, 29(2):153-158.
[5] Keinath A P. Sensitivity of populations of Phytophthora capsici from South Carolina to mefenoxam, dimethomorph, zoxamide and cymoxanil[J]. Plant Disease An International Journal of Applied Plant Pathology, 2007, 91(6):743-748.
[6] Lee Y S, Kim J, Shin S C, et al. Antifungal activity of Myrtaceae essential oils and their components against three phytopathogenic fungi[J]. Flavour and Fragrance Journal, 2008, 23(1):23-28.
[7] Kousik C S, Keinath A P. First report of insensitivity to cyazofamid among isolates of Phytophthora capsici from the Southeastern United States[J]. Plant Disease An International Journal of Applied Plant Pathology, 2008, 92(6):979-979.
[8] Pang Z, Shao J, Chen L, et al. Resistance to the novel fungicide pyrimorph in Phytophthora capsic:risk assessment and detection of point mutations in CesA3 that confer resistance[J]. PLoS ONE, 2013, 8(2):e56513.
[9] Quesada-Ocampo L M, Hausbeck, M K. Resistance in tomato and wild relatives to crown and root rot caused by Phytophthora capsici[J]. Phytopathology, 2010, 100(6):619.
[10] Dan Y, Liu H Y, Gao W W, et al. Activities of essential oils from Asarum heterotropoides, var. mandshuricum, against five phytopathogens[J]. Crop Protection, 2010, 29(3):295-299.
[11] Hashem M, Moharama A M, Zaied A A, et al. Efficacy of essential oils in the control of cumin root rot disease caused by Fusarium spp[J]. Crop Protection, 2010, 29(10):1111-1117.
[12] Zhao T, Gao F, Zhou L, et al. Essential oil from Inula britannica extraction with SF-CO2 and its antifungal activity[J]. Journal of Integrative Agriculture, 2013, 12(10):1791-1798.
[13] Ma B X, Ban X Q, He J S, et al. Antifungal activity of Ziziphora clinopodioides Lam. essential oil against Sclerotinia sclerotiorum on rapeseed plants (Brassica campestris L.)[J]. Crop Protection, 2016, 89(11):289-295.
[14] Thomidis T, Filotheou A. Evaluation of five essential oils as bio-fungicides on the control of Pilidiella granati rot in pomegranate[J]. Crop Protection, 2016, 89(1):66-71.
[15] Varo A, Mulero-Aparicio A, Adem M, et al. Screening water extracts and essential oils from Mediterranean plants against Verticillium dahliae in olive[J]. Crop Protection, 2017, 92(2):168-175.
[16] 唐裕芳, 张妙玲, 黄白飞. 肉桂油的提取及其抑菌活性研究[J]. 天然产物研究与开发, 2006, 18(3):432-434.
[17] 董红平, 赵特, 孙淑君, 等. 肉桂精油对4种重要果蔬病原真菌的生物活性研究[J]. 中国生物防治学报, 2014, 30(2):282-286
[18] 杜小琴. 植物精油对甜樱桃采后病原真菌的抑制作用及其贮藏效果研究[D]. 四川农业大学, 2016.
[19] 房耀欣. 精油对柑橘采后致腐真菌的抑菌活性及主要成分研究[D]. 南京大学, 2017.
[20] 吴建挺, 张悦丽, 张博, 等. 11种植物精油对6种植物病原真菌的抑菌活性研究[J]. 植物科学学报, 2013, 31(2):198-202.
[21] 解淑慧, 邵兴锋, 王可, 等. 柑橘采后腐烂主要致病菌的分离鉴定及丁香精油对其抑制作用研究[J]. 果树学报, 2013, 30(1):134-139.
[22] 刘丽, 胡梁斌, 王德德, 等. 肉桂醛对辣椒疫霉的抑制作用[J]. 江苏农业学报, 2014, 30(2):282-288.
[23] 薛延丰, 陈健, 张猛, 等. 肉桂醛对辣椒生物量和品质影响及辣椒疫病防控效果初探[J]. 西南农业学报, 2014, 27(2):781-787.
[24] 慕立义, 吴文君, 王开运. 植物化学保护研究方法[M]. 北京:中国农业出版社, 1994, 79-81.
[25] Matheron M E, Porchas M. Impact of azoxystrobin, dimethomorph, fluazinam, fosetyl-A1, and metalaxyl on growth, sporulation, and zoospore cyst germination of three Tophthora spp.[J]. Plant Disease, 2000, 84(4):454-458.
[26] Hamm P B, Cummings T F, Johnson D A. Comparison of deposition patterns in two programs for applying protectant fungicides to potato stems and leaves for the control of late blight[J]. American Journal of Potato Research, 2006, 83(6):473-484.
[27] 崔晓岚, 孟庆晓, 毕扬, 等. 辣椒疫霉对烯酰吗啉的敏感性基线及室内抗药突变体研究[M]. 植物病理学报, 2009, 39(6):630-637.
[28] 马辉刚, 何烈干, 张海良, 等. 江西省辣椒疫霉生理小种构成及其对烯酰吗啉的敏感性分析[J]. 植物保护学报, 2013, 40(4):374-378.
[29] 彭化贤, 刘波微, 李薇. 四川辣椒疫霉菌生物学特性和辣椒抗霉疫病性鉴定方法初探[J]. 云南农业大学学报, 2005, 20(1):140-144.
[30] 王进强, 许文耀, 朱巧灵, 等. 56%氢氧化铜·烯酰吗啉可湿性粉剂对辣椒疫菌的防治效果[J]. 亚热带农业研究, 2006, 2(1):37-39.
[31] Kaul P N, Bhattacharya A K, Rajeswara Rao B R, et al. Volatile constituents of essential oils isolated from different parts of cinnamon (Cinnamomum zeylanicum Blume)[J]. Journal of the Science of Food and Agriculture, 2003, 83(1):53-55.
[32] Cheng S S, Liu J Y, Hsui Y R, et al. Chemical polymorphism and antifungal activity of essential oils from leaves of different provenances of indigenous cinnamon (Cinnamomum osmophloeum)[J]. Bioresource Technology, 2006, 97(2):306-312.
[33] 李丽娜, 石志琦, 高弢, 等. 肉桂醛对增强辣椒疫霉病抗性的作用机制研究[J]. 西北植物学报, 2016, 36(1):100-105.