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Chinese Journal Of Biological Control ›› 2020, Vol. 36 ›› Issue (2): 175-183.DOI: 10.16409/j.cnki.2095-039x.2020.02.003

• RESEARCH REPORTS • Previous Articles     Next Articles

Laboratory Toxicity and Field Control Efficacy of Biopesticide Bacillus thuringiensis G033A on the South American Tomato Leafminer Tuta absoluta (Meyrick), a New Invasive Alien Species in China

ZHANG Guifen1,2, ZHANG Yibo1,2, ZHANG Jie1, LIU Wanxue1,2, WANG Yusheng1, WAN Fangfao1,2, SHU Changlong1, LIU Hui3, WANG Fulian4, ZHAO Lin1,4, LI Qinghong5, WANG Shuming6, JIANG Jiaqiang7   

  1. 1. State Key Laboratory for Biology of Plant Diseases and Insect Pests/Key Laboratory of Integrated Pest Management of Crop, Ministry of Agriculture and Rural Affairs of the People's Republic of China/Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
    2. Center for Management of Invasive Alien Species, Ministry of Agriculture and Rural Affairs of the People's Republic of China, Beijing 100193, China;
    3. The National Agro-Tech Extension and Service Center, Beijing 100026, China;
    4. Yangtze University, Jingzhou 434100, China;
    5. Yunnan Plant Protection and Quarantine Station, Kunming 650034, China;
    6. Yuxi Plant Protection and Quarantine Station, Yuxi 653100, China;
    7. Yuxi Yangrui Agricultural Development Co., Ltd, Yuxi 653100, China
  • Received:2019-09-13 Online:2020-04-08 Published:2020-04-16

Abstract: The South American tomato leafminer Tuta absoluta (Meyrick), a world destructive tomato insect pest, is a new invasive alien species in China and has posed a potential threat to the Chinese tomato industry. To control T. absoluta effectively, the laboratory toxicity and field control efficacy of Bacillus thuringiensis G033A (Bt G033A, effective component, 32000 IU/mg), a newly developed bivalent genetically engineered bacteria, were evaluated using leaf dipping and field spraying methods, respectively. Laboratory toxicity was the highest on the 4th instar larvae of T. absoluta, decreased in the 3rd instars and further decreased in the 1stto 2nd instars, as indicated by LC50 of 14.63, 15.59 and 23.17 g/L, respectively. Bt G033A had high control efficacy on T. absoluta larvae when the concentration of Bt G033A was 10 g/L or above, with a corrected mortality of over 90% at 96 h post treatment. On tomato plants grown in greenhouses, 100-fold diluents (10 g/L) of Bt G033A WP had high control efficacy on young larvae of T. absoluta. The corrected mortalities to the 1st and 2nd instars were 89.1% and 98.0%, respectively, on the fifth day after treatment and reached 100% on the seventh day after treatment. The insecticide activity of Bt G033A is comparable to that of 5% the Rotenone. The results can benefit the management of the South American tomato leafminer in organic vegetable production bases.

Key words: Tuta absoluta, Bacillus thuringiensis, indoor toxicity, field control efficacy

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