Isolation and Identification of Coprophilous Chaetomium Strain and Its Biocontrol Effect against Pepper Fusarium Wilt

doi:10.16409/j.cnki.2095-039x.2017.04.017

Abstract

Abstract: The seventy-six coprophilous fungal strains were isolated from Pepper rhizosphere against Fusarium oxysporum by using dual-culture and inhibition zone assay. Among them, strain LJ-S2L1 with prominent potential was obtained on the basis of evaluation by colony diameter inhibition assay, Kirby-Bauer, toxicity measurement and pot experiments. The results showed that the inhibitory rate of LJ-S2L1 against F. oxysporum was 71.67%. The hyphae of pathogen was collapsed and fractured, and the diameter of inhibition zone was 32 mm by the inhibition zone assay. Both sterilized and non-sterilized fermentation liquid of LJ-S2L1 were effective against pathogen hyphae, and the inhibitory rates were 61.57% and 56.48%, respectively. In toxicity measurement, the inhibitory rate of LJ-S2L1 fermentation was 72.10% at the concentration of 1000 μL/mL, and the effective concentration (EC50) was 300.63 μL/mL. Pot trials showed that strain LJ-S2L1 effectively controlled pepper wilt with the inhibitory rate of 71.8%. Strain LJ-S2L1 was identified as Chaetomium globosum on the basis of the morphological characteristics and phylogenetic tree.

Key words: Fusarium oxysporum, coprophilous fungus, hyphal dissolving, Chaetomium, biological control

CLC Number:

S476

MAO Tingting, TAO Gang, ZHAO Dailin, ZHAO Xingli, LIANG Yanping, WANG Nian, GU Jingang. Isolation and Identification of Coprophilous Chaetomium Strain and Its Biocontrol Effect against Pepper Fusarium Wilt[J]. journal1, 2017, 33(4): 552-560.

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