Screening and Control Efficiency Evaluation of Bacillus against Rice Blast Magnaporthe oryzae

doi:10.16409/j.cnki.2095-039x.2018.03.008

Abstract

Abstract: 232 bacterial strains were isolated from rice rhizosphere of cultivar Ningjin NO. 48 in Ningxia Hui Autonomous Region. Only two isolates, S09 and S170 strongly inhibited the growth of Magnaporthe oryzae in dual culture technique and leaf blade in vitro. The hyphal growth inhibition rate of strains S09 and S170 on M. oryzae cultured after 4 d were 90.44% and 92.38%, respectively. The control efficacies in leaf blades were 74.62% and 75.52%, respectively, without significant difference from that of Lvdikang NO. 1. 16S rDNA sequence analysis indicated that strain S09 had the highest homology with B. pumilus. gyrA gene sequence analysis indicated that S170 had the highest homology with B. amyloliquefacien. Combining with its the morphological characteristic, the strains S09 and S170 were identified as B. pumilus and B. amyloliquefaciens, respectively. The control efficacies of strains S09 and S170 against rice blast were 76.56% and 80.57% under greenhouse condition, respectively. Field trials showed that strains S09 and S170could promote rice growth, increase the germination rate by 17.37% and 12.34%, promote rice root length by 18.68% and 33.85%, enhance rice plant height by 12.44% and 28.49%, and reduce empty-grain percentage by 25.17% and 35.59%, respectively. Moreover, both trains could improve chalky grain rate and chalkiness ratio. Field control efficacies of B. pumilus S09 and B. amyloliquefaciens S170 were 70.59% and 73.19% against leaf blast, and 71.55% and 74.82% against neck blast, respectively. The control efficacy of strain S170 had no significant difference with that of 75% tricyclazole wettable powder or Lvdikang NO. 1.

Key words: rice blast, Bacillus, screen, bio-control, field efficacy

CLC Number:

S476

SHA Yuexia, ZENG Qingchao, WANG Xin, SHEN Ruiqing, LIU Hao, WANG Xigang. Screening and Control Efficiency Evaluation of Bacillus against Rice Blast Magnaporthe oryzae[J]. journal1, 2018, 34(3): 414-422.

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