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Chinese Journal Of Biological Control ›› 2020, Vol. 36 ›› Issue (2): 265-271.DOI: 10.16409/j.cnki.2095-039x.2020.02.01

• RESEARCH REPORTS • Previous Articles     Next Articles

Control Effect of Mixed Inoculation of Different Biocontrol Strains on Botrytis cinerea

WANG Hongli1, SHAN Wenhui1, HU Haiyao1, LI Yan1, WANG Qi1, WANG Kun2, BIAN Fenge2   

  1. 1. Laboratory of Plant Pathology, China Agricultural University, Beijing 100193, China;
    2. Chateau Junding, Co. Ltd, Penglai 265600, China
  • Received:2019-06-27 Online:2020-04-08 Published:2020-04-16

Abstract: Inhibition effect of Bacillus subtilis GLB191 and B. pumilus GLB197 single strain and mixed strains against B. cinerea was investigated by inhibition zone test, inhibition test of conidia germination and in vitro inoculation on fruit. The results showed that the inhibition zone size of each treatment was more than 30 mm, and the inhibition rate of spore germination was more than 94%. The mixture of the two strains with 500 times dilution inhibited the spore germination higher than single strain. In vitro inoculation on fruit test showed that the lesion inhibition rate of the mixed strains treatment was up to 70.89%,higher than that of the single strain GLB191 and GLB197, which was 55.48% and 52.91%, respectively. Field trials indicated that control efficiency of the mixed strains was no significant difference with chemical agents, with the efficacy of 70.02%. Therefore, mixed inoculation of the two strains can be applied in grape grey mold control.

Key words: Botrytis cinerea, Bacillus, mixed inoculation, inhibitory effect, field efficacy

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