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journal1 ›› 2018, Vol. 34 ›› Issue (5): 771-778.DOI: 10.16409/j.cnki.2095-039x.2018.05.017

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Composition of Bacterial Community in Maize Rhizosphere and Screening of Biocontrol Bacteria Strains

LIU Quancheng1, ZHANG Xixi1, TIAN Xueliang2, LIU Wende1   

  1. 1. State Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
    2. Henan Institute of Science and Technology, Xinxiang 453003, China
  • Received:2018-03-15 Online:2018-10-08 Published:2018-10-15

Abstract: To clarify composition bacterial community in maize rhizosphere and screen biocontrol resources for maize diseases, a total of 1584 bacteria strains were isolated from maize rhizosphere by culture-dependent method. The bacteria were classified into Proteobacteria, Actinobacteria, Firmicutes and Bacteroidetes, among which Proteobacteria were the dominant groups. At genus level, Pseudomonas was the most abundant groups. A total of 162 bacteria strains with antagonism against Setosphearia turcica were selected through plate confrontation method and strain D3 had the strongest antifungal effect. Moreover, strain D3 was identified as Bacillus amyloliquefaciens by morphological and molecular methods. Strain D3 had broad-spectrum antifungal effect on maize fungal pathogens, including S. turcica, Colletotrichum graminicola, Fusarium avenaceum and Curvularia lunata. Using the specific primers of lipopeptide gene, the related genes could be amplified by PCR in strain D3. Additionally, cell-free filtrate and the crude extract of lipopeptide from strain D3 had biocontrol effect on pathogen with the inhibition rate of 81.56%. In conclusion, strain D3 can be used as a new biocontrol microorganism against maize fungal diseases.

Key words: rhizosphere microbes, culturable bacteria, Bacillus amyloliquefaciens, biological control, maize

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