Welcome to Chinese Journal of Biological Control,Today is

journal1 ›› 2019, Vol. 35 ›› Issue (1): 81-88.DOI: 10.16409/j.cnki.2095-039x.2019.01.017

Previous Articles     Next Articles

Culture Optimization for Fermentation of Avirulent Ralstonia Solanacearum Strain FJAT-1458 Used as Plant Vaccine against Crop Bacterial Wilt

CHEN Yanping, XIAO Rongfeng, LIU Bo, ZHU Yujing, ZHENG Xuefang, CHEN Deiju   

  1. Institute of Agricultural Biological Resource Research, Fujian Academy of Agricultural Sciences, Fuzhou 350003, China
  • Received:2018-06-11 Online:2019-02-08 Published:2019-01-31

Abstract: FJAT-1458 is an avirulent Ralstonia solanacearum strain used as plant vaccine against crop bacterial wilt disease. In order to improve the fermentation level of the strain FJAT-1458, the liquid and solid fermentation culture media and culture conditions were optimized by using single factor and orthogonal test analysis. The solid-state fermentation medium was mainly the litters from microbial fermentation bed for pig-raising, and the bacterial yield was used as the indicator in all tests. The results showed that optimal liquid fermentation medium formula for the strain was corn flour 2.5%, fishbone meal 0.5%, sucrose 0.1%, peptone 0.15%, K2HPO4 0.05% and MgSO4 0.025%. The average bacterial yield of FJAT-1458 could reach up to 3.87×109 cfu/mL for 48 h. The optimal solid fermentation medium formula for the strain was a mass ratio of 7:3 for litters and soybean powder. The optimal fermentation conditions were temperature 35℃, relative moisture 45%, inoculation quantity 15%, ventilation 70% and fermentation time for 40 h. The average bacterial yield of strain FJAT-1458 could reach up to 4.68×108 cfu/g.

Key words: plant vaccine, liquid fermentation, solid fermentation, medium optimization, culture condition optimization

CLC Number: