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Chinese Journal Of Biological Control ›› 2019, Vol. 35 ›› Issue (4): 586-596.DOI: 10.16409/j.cnki.2095-039x.2019.04.016

• RESEARCH REPORTS • Previous Articles     Next Articles

Optimization of Solid Fermentation Conditions of Bacillus subtilis 262XY2x and Its Control Effect on Colletotrichum coccodes

LIU Zhihui, YANG Chengde, JIN Mengjun, LI Tonghua, CHEN Xingyi, WEI Lijuan, CUI Lingxiao, YANG Liping   

  1. College of Plant Protection, Gansu Agricultural University/Biocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province, Lanzhou 730070, China
  • Received:2019-01-11 Online:2019-08-08 Published:2019-08-10

Abstract: Bacillus subtilis 262XY2xwas used as antagonistic bacteria in this experiment. The solid fermentation matrix of B. subtilis 262XY2x was screened by one-way analysis of variance (ANOVA) and orthogonal combination test, optimized the fermentation conditions and the function of disease control and promoting growth were determined. The results showed that the optimal medium for solid fermentation of B. subtilis 262XY2x contained bran (36.4%), rice hull powder (31.8%), corn gluten (18.2%), soybean meal (13.6%), lactose (1.0%), urea (1.1%) and ZnSO4 (40.11%). The viable amounts was up to 7.13×1014 CFU/g in the optimal fermentation conditions which contained inoculum 10%, solid content 44 g/L, feed water ratio 1:1.4, fermentation temperature 28℃, fermentation initial pH was 7.0 and fermentation time was 40-44 h. When the dosage of the microbial agent was 1.0%, the plant height and root length of the potato were significantly higher than that of the control (P<0.05), and the growth ratios were 45.25% and 28.43%, respectively. The PAL activity in the potato was significantly increased compared with the control (P<0.05) 16.57%, and the preventive effect on Colletotrichum coccodes and the therapeutic effect were 63.45% and 46.70%.

Key words: Bacillus subtilis, solid fermentation, optimization, Colletotrichum coccodes

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