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journal1 ›› 2017, Vol. 33 ›› Issue (2/3): 385-393.DOI: 10.16409/j.cnki.2095-039x.2017.03.013

• RESEARCH REPORTS • Previous Articles     Next Articles

Effects of Avirulent Ralstonia solanacearum Strain as Plant Vaccine on Microbial Community in Rhizosphere Soil of Tomato

ZHENG Xuefang, LIU Bo, ZHU Yujing   

  1. Agricultural Bio-Resources Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350003, China
  • Received:2016-12-29 Online:2017-06-08 Published:2017-04-08

Abstract: In order to explore the effect of avirulent Ralstonia solanacearum strain FJAT-1458 as plant vaccine on microbial community structures in tomato rhizosphere soil, tomato plants were inoculated by FJAT-1458 with water as a control. Then the rhizosphere soil was sampled at 3, 6, 9, 12, 15, 18 and 21 d after treatment, respectively. Phospholipid fatty acids (PLFAs) of soil samples were detected using Agilent 6890 N. The results showed that PLFAs composition and content in tomato rhizosphere soil were significantly altered by inoculation of FJAT-1458, which could be divided into decreasing type, unchanging type and increasing type. Further statistical analysis showed that the inoculation of FJAT-1458 could increase the content of total microbes, bacteria and fungi by 5.98%-47.36%, 2.07%-58.07% and 5.65%-74.42%, respectively. Growth of actinomycetes was inhibited at the beginning and then improved by FJAT-1458 inoculation. Moreover, inoculation of FJAT-1458 could increase the microbial community diversity in tomato rhizosphere soil, and the enhancement of Shannon-wiene index was the most significant, with the highest value of 13.32% at 21 d after inoculation. Cluster analysis revealed that the microbe in tomato rhizosphere soil both in FJAT-1458 treatment and control could be divided into three communities at 3.28 and 4.62 Lance distance, but constitutes and characters of PLFAs between the two treatments were different. Principal component analysis demonstrated that the two treatments could also be distinguished into two different groups. All these suggested that inoculation of FJAT-1458 could alter microbial community structure in tomato rhizosphere soil and increase its diversity, and further improve the stability of soil micro-ecosystem and the ability for bacterial wilt disease control.

Key words: plant vaccine, tomato, soil microbial community structure, PLFAs

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