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journal1 ›› 2019, Vol. 35 ›› Issue (3): 463-473.DOI: 10.16409/j.cnki.2095-039x.2019.03.016

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Genome Sequencing and Identification of Chitinase Family Genes of Streptomyces sp. FT05W, an Antagonist of Tobacco Pathogens

QIN Ke1, SANG Weijun2, CHEN Xiaoyulong2, LI Haoxi2, YANG Maofa2,3   

  1. 1. Agricultural College of Guizhou University, Guiyang 550025, China;
    2. Tobacco College of Guizhou University, Guiyang 550025, China;
    3. Guizhou Key Laboratory for Plant Pest Management of Mountainous Region/Institute of Entomology, Guiyang 550025, China
  • Received:2018-10-26 Online:2019-06-08 Published:2019-06-17

Abstract: Streptomyces sp. FT05W was a biocontrol strain with antagonism to tobacco soil-borne fungal pathogens. In this study, we used Miseq PE300 next-generation sequencing platform to perform the genome sequencing, and obtained 6914188 reads. The SPAdes software was used to splice a total of 1836 contigs with a total genome length of 7699129 bp and a GC ratio of 71%. Length of the coding gene sequence was 6037181 bp, and 7434 protein-coding genes were predicted, which are predicted to encode 7233 proteins. Subsequently, through the comparison and analysis of databases such as GO, COG, KEGG, NR and TCDB, the genome prediction and basic function annotations were completed. The obtained gene annotation information lays an important foundation for further research on its biological control mechanism in tobacco. The results will help to promote the excavation and utilization of Streptomyces sp. functional genes and provide a theoretical basis for further development and application of Streptomyces sp. FT05W. Finally, the chitinase family gene phylogenetic tree of Streptomyces sp. FT05W was constructed using MEGA 6.06. A total of seven chitinase gene families were identified. Six were family18 chitinase genes and one was family 19 gene.

Key words: tobacco biocontrol strain, Streptomyces sp., genome sequencing, chitinase family gene

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