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Chinese Journal Of Biological Control ›› 2020, Vol. 36 ›› Issue (5): 744-750.DOI: 10.16409/j.cnki.2095-039x.2020.05.013

• RESEARCH REPORTS • Previous Articles     Next Articles

Virulence of a Nucleopolyhedrovirus, SeMNPV-QD, Isolated from Spodoptera exigua in Qingdao, China

SUN Yu1, CHEN Yingjian1, YANG Mingxin2, ZHEN Guiling1, HAN Jiachen1, LI Jie1, LI Changyou1   

  1. 1. College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao 266109, China;
    2. Qingdao City-farmer Agricultural Technology Co. Ltd., Qingdao 266109, China
  • Received:2020-01-07 Online:2020-10-08 Published:2020-11-20

Abstract: Biocontrol potential of a new nucleopolyhedrovirus isolated from Spodoptera exigua in Qingdao (SeMNPV-QD) was evaluated by measuring the laboratory bioactivity and field control efficacy of SeMNPV-QD and SeMNPV American isolate (SeMNPV-US1). The results showed that the infection rates of SeMNPV-QD and SeMNPV-US1 to the S. exigua cell line Se-3 were 92.34% and 93.65%, respectively, and the yield of virus polyhedra was 23.97 PIB/cell and 24.10 PIB/cell, respectively. There were no significant differences in infection rate and yield of virus polyhedra between the two virus isolates. The LC50 of SeMNPV-QD and SeMNPV-US1 against the first instar S. exigua larvae were 4.43×104PIB/mL and 4.35×104 PIB/mL, respectively, and the LT50 were 4.12 d and 4.02 d, respectively. The LC50 against the fourth instar S. exigua larvae were 9.25×105PIB/mL and 4.44×105 PIB/mL, respectively, and the LT50 were 6.20 d and 5.50 d, respectively. SeMNPV-QD and SeMNPV-US1 achieved high control, 74.99% and 79.04%, respectively, against S. exigua larvae on green Chinese onion. These results provide theoretical basis for further research and development of this new virus isolate and biological control of S. exigua.

Key words: Spodoptera exigua, nucleopolyhedrovirus, infection rate, bioassay, control efficiency

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