Virulence of a Nucleopolyhedrovirus, SeMNPV-QD, Isolated from Spodoptera exigua in Qingdao, China

doi:10.16409/j.cnki.2095-039x.2020.05.013

Abstract

Abstract: Biocontrol potential of a new nucleopolyhedrovirus isolated from Spodoptera exigua in Qingdao (SeMNPV-QD) was evaluated by measuring the laboratory bioactivity and field control efficacy of SeMNPV-QD and SeMNPV American isolate (SeMNPV-US1). The results showed that the infection rates of SeMNPV-QD and SeMNPV-US1 to the S. exigua cell line Se-3 were 92.34% and 93.65%, respectively, and the yield of virus polyhedra was 23.97 PIB/cell and 24.10 PIB/cell, respectively. There were no significant differences in infection rate and yield of virus polyhedra between the two virus isolates. The LC₅₀ of SeMNPV-QD and SeMNPV-US1 against the first instar S. exigua larvae were 4.43×10⁴PIB/mL and 4.35×10⁴ PIB/mL, respectively, and the LT₅₀ were 4.12 d and 4.02 d, respectively. The LC₅₀ against the fourth instar S. exigua larvae were 9.25×10⁵PIB/mL and 4.44×10⁵ PIB/mL, respectively, and the LT₅₀ were 6.20 d and 5.50 d, respectively. SeMNPV-QD and SeMNPV-US1 achieved high control, 74.99% and 79.04%, respectively, against S. exigua larvae on green Chinese onion. These results provide theoretical basis for further research and development of this new virus isolate and biological control of S. exigua.

Key words: Spodoptera exigua, nucleopolyhedrovirus, infection rate, bioassay, control efficiency

CLC Number:

S476.1

SUN Yu, CHEN Yingjian, YANG Mingxin, ZHEN Guiling, HAN Jiachen, LI Jie, LI Changyou. Virulence of a Nucleopolyhedrovirus, SeMNPV-QD, Isolated from Spodoptera exigua in Qingdao, China[J]. Chinese Journal Of Biological Control, 2020, 36(5): 744-750.

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