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Chinese Journal Of Biological Control ›› 2020, Vol. 36 ›› Issue (1): 31-39.DOI: 10.16409/j.cnki.2095-039x.2020.01.004

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Physiochemical Changes in Diapause Prepupae of the Green Lacewing Chrysopa formosa

WANG Manzi1,2, LI Yuyan2, GAO Fei2, LIU Mengyao1,2, ZHANG Hongzhi2, XU Cheng2, ZANG Liansheng1, ZHANG Lisheng1,2   

  1. 1. Faculty of Agronomy Jilin Agricultural University, Jilin 130117, China;
    2. USDA-ARS Sino-American Biological Control Laboratory/Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2019-02-27 Online:2020-02-08 Published:2020-02-26

Abstract: The green lacewing Chrysopa formosa is an important predator of a variety of agricultural and forestry insect pests. It enters facultative diapause as prepupa in cocoon, which offers a natural stage that can be used to elongate shelf life, increase product stock and promote long-distance shipment. In this study, we measured the contents of key biochemical substances such as total protein, lipids, sugars and alcohols, and the activities of enzymes such as lipase, trehalase and antioxidant enzymes (superoxide dismutase, peroxidase and catalase) in non-diapause prepupae (1 d old) and diapause prepupae (1 d and 7 d old) of C. formosa. Differences in these biochemical markers among the three treatments were analyzed. Our results indicated that the diapause and non-diapause prepupae were physiochemically different. Diapause prepupae accumulated more energy reserves, including proteins, lipids, triacylglyceride and glycogen, as well as cryprotectants, such as glycerol and trehalose. Further, the anti-oxidation ability was highly increased in the diapause prepupae. These physiochemical changes in diapause prepupae can contribute to enhance their stress tolerance, meet the energy requirements for diapause maintenance and post-diapause development, and increase survival throughout diapause. The results provide information for further understanding physiological mechanism of diapause in the green lacewings.

Key words: Chrysopa formosa, diapause, lipids, sugar alcohols, enzymatic activity

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