Identification of New Virus Strain of Apocheima cinerarius and Its Synergistic Effect on A. cinerarius Nucleopolyhedrovirus

doi:10.16409/j.cnki.2095-039x.2020.02.005

Abstract

Abstract: Apocheima cinerarius (Erschoff) is one of the major insect pests in China. A new virus strain that can infect the larvae of A. cinerarius was isolated and purified, and measured for its virulence and synergistic effect to A. cinerarius nucleopolyhedrovirus (ApciNPV). The ultrastructural observation of the new strain showed that the virus was mostly of a hexagonal polyhedron, with a diameter of 0.32~1.20 μm, and with nearly spherical virion. With the structural features of a cypovirus, the virus was named as ApciCPV. Biological activity assays showed that ApciCPV was virulent against the 3^rd instar larvae of A. cinerarius, with LC₅₀ of 1.42×10⁴ OBs/mL. Interestingly, ApciCPV showed a synergistic effect to ApciNPV. When the 3^rd instar larvae of A. cinerarius were exposed to ApciCPV in combination with ApciNPV, LT₅₀ of ApciNPV to the larvae was significantly shorten by 0.50-10.90 d, and the final lethal rate of ApciNPV increased by 6.70%-43.30%. Based on mortality, LT₅₀ and ST, the mixed solution of 1.0×10⁶ OBs/mL ApciNPV and 1.0×10⁴ OBs/mL ApciCPV, has the most virulence against the 3^rd instar larvae of A. cinerarius. These results show that the newly discovered viral strain, ApciCPV, is one of the ideal biological agents to control A. cinerarius.

Key words: A. cinerarius cypovirus, A. cinerarius nucleopolyhedrovirus, Apocheima cinerarius, synergistic effect

CLC Number:

S476.1

WANG Chengrui, LI Enjie, WANG Shaobo, WANG Qinghua, WANG Yuzhu, ZHANG Yongan, DUAN Liqing. Identification of New Virus Strain of Apocheima cinerarius and Its Synergistic Effect on A. cinerarius Nucleopolyhedrovirus[J]. Chinese Journal Of Biological Control, 2020, 36(2): 196-202.

References

[1] 庞竟公, 何稳稳, 曹玉, 等. 重要林木害虫春尺蠖研究进展[J]. 农业科学, 2018, 8(5):461-467.
[2] 张明华. 绥化地区杨树防护林带主要虫害的防治[J]. 现代农村科技, 2019(3):24.
[3] 乔丽霞. 邢台市春尺蠖的发生与防治[J]. 现代农村科技, 2018(6):20.
[4] 臧健. 安徽淮北地区春尺蠖风险分析[J]. 安徽林业科技, 2017(3):29-31, 36.
[5] Moscardi F. Assessment of the application of baculoviruses for control of Lepidoptera[J]. Annual Review of Entomology, 1999, 44:257-89.
[6] Boguslaw S, Lukasz R, Ewelina K, et al. Baculovirus biopesticides-a safe alternative to chemical protection of plants[J]. Journal of Biopesticides, 2009, 2(2):209-216.
[7] 张永安, 王贵成, 徐保泯, 等. 新疆和田地区利用AciNPV病毒防治春尺蠖试验报告[J]. 新疆林业, 2002(2):20-21, 30.
[8] 冯宏祖, 王兰, 胡卫江, 等. 沙雅县天然胡杨林春尺蠖发生及飞防效果分析[J]. 中国森林病虫, 2005, 24(1):22-24.
[9] 李颖, 孙兴鲁, 浦冠勤. 昆虫杆状病毒杀虫剂的研究进展[J]. 中国蚕业, 2010, 31(4):12-14.
[10] 薛贵收, 肖娱玉, 朱江, 等. 昆虫杆状病毒杀虫剂的研究与应用进展[J]. 江苏农业科学, 2009(2):8-11.
[11] Tanada Y. Synergism between two viruses of the armyworm, Pseudaletia unipuncta (Haworth) (Lepidoptera, Noctuidae)[J]. Insect Pathology, 1959, 1(2):215- 231.
[12] 王晓容, 匡石滋, 吴洁芳, 等. 2种昆虫病毒混合悬液对菜粉蝶的增效作用[J]. 华中农业大学学报, 2005, 24(1):21-24.
[13] Derksen A C, Granados R R. Alteration of a lepidopteran peritrophic membrane by baculoviruses and enhancement of viral infectivity[J]. Virology, 1988, 167(1):242-250.
[14] Gallo L G, Corsaro B G, Hughes P R, et al. In vivo enhancement of baculovirus infection by the viral enhancing factor of a granulosis virus of the cabbage looper, Trichoplusia ni (Lepidoptera, Noctuidae)[J]. Journal of Invertebrate Pathology, 1991, 58(2):203-210.
[15] 郭慧芳, 方继朝, 韩召军. 昆虫病毒增效剂研究进展[J]. 昆虫学报, 2003, 46(6):766-772.
[16] 陈立坤, 张永安, 王玉珠, 等. Bt和LdNPV混用对舞毒蛾幼虫毒杀效果的研究[J]. 中国森林病虫, 2013, 32(3):36-39, 35.
[17] 白小东, 丁翠. 黄地老虎NPV增效作用的研究[J]. 应用于环境生物学报, 2000, 6(1):52-55.
[18] 王燕, 丁翠, 蔡秀玉. 混合感染后杆状病毒间的增效作用——病毒蛋白及核酸的初步分析[J]. 昆虫学报, 1997, 40(1):7-14.
[19] 徐健, 刘琴, 祝树德. 昆虫颗粒体病毒增效蛋白研究进展[J]. 华东昆虫学报, 2005, 14(4):343-347.
[20] Xu J H, Tosihiko H. Enhanced infection of a nuclear polyhedrosis virus in larvae of the armyworm, Pseudaletia separata, by a factor in the spheroids of an entomopoxvirus[J]. Journal of Invertebrate Pathology, 1992, 60(3):259-264.
[21] Shapiro M. Effect of two granulosis viruses on the activity of the gypsy moth (Lepidoptera:Lymantriidae) nuclear polyhedrosis virus[J]. Journal of Economic Entomology, 2000, 93(6):1633-1637.
[22] 曲良建, 王玉珠, 陶婧, 等. 春尺蠖NPV生物学特性及室内增殖研究[J]. 中国森林病虫, 2011, 31(6):35-37.
[23] 李海龙, 张桂林, 党荣理, 等. 应用SPSS软件进行杀虫剂的KT50分析[J]. 中国媒介生物学及控制杂志, 2011, 22(2):155-157.
[24] Lobinger G. On the synergism of a cytoplasmic polyhedrosis virus (DpCPV) isolated from Dasychira pudibunda L. (Lep., Lymantriidae) in mixed infections with different nuclear polyhedrosis viruses[J]. Journal of Applied Entomology-Zeitschrift Fur Angewandte Entomologie, 1991, 112(4):335-340.
[25] 郭慧芳, 方继朝, 罗伟杰, 等. 不同昆虫病毒对斜纹夜蛾和甜菜夜蛾的联合增效作用[J]. 中国生物防治, 2003, 19(1):23-26.