Analysis and Molecular Detection of Isaria cicadae Mating-type Gene

doi:10.16409/j.cnki.2095-039x.2015.06.007

Abstract

Abstract: The filamentous fungus Isaria cicadae is known as the anamorph of medicinal fungi Cordyceps cicadae and is widely used for biological control against homoptera pests. Although I. cicadae is widespread in the wild, Isaria cicadae is present mainly in the form of coremium and finding sexual development of this fungus is extremely difficult because of its obscure sexual stages. The mating system of fungi is a determining factor for sexual reproduction, but mating-type (MAT) genes of I. cicadae have not been previously investigated. Therefore, the presence and functionality of MAT genes in the fungi were investigated to assess the potential for sexual reproduction in this study. Firstly, the full-length cDNA of MAT1-1-1 and MAT1-2-1 were cloned from I. cicadae using RACE technology, and the characteristics of the genes were analyzed. Furthermore, based on the full-length cDNA, PCR primers set for the MAT1-1-1 and MAT1-2-1 were designed and optimized, respectively. And the mating type of 40 strains from Anhui province were determined to be MAT1-1 (14 strains) were identified while the others were MAT1-2 (26 strains), with no deletion or a dominant mating type strains in the distribution. These results suggested that other elements, such as growing environment or hereditary factors, may contribute to the obscure sexual fruiting-body instead of mating type.

Key words: mating-type gene, Isaria cicadae, gene cloning

CLC Number:

S476.12

GAO Yang, ZHANG Xi, HUANG Bo. Analysis and Molecular Detection of Isaria cicadae Mating-type Gene[J]. journal1, 2015, 31(6): 860-867.

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