Function of Short-chain Dehydrogenase Gene CrSdr in Clonostachys rosea 67-1

doi:10.16409/j.cnki.2095-039x.2021.06.001

Abstract

Abstract: A differentially expressed short-chain dehydrogenase gene, CrSdr, was identified from the transcriptome of Clonostachys rosea 67-1 mycoparasitizing the sclerotia of Sclerotinia sclerotiorum, which was previously constructed in our lab. Quantitative real-time PCR detection showed that the expression level of CrSdr increased 4 times after the induction of sclerotia for 24 h. Gene knock-out and complementation were conducted to investigate the function of CrSdr. The results showed that the gene-deleted mutants had the same growth speed with the wild type, spore yields increased by 43.2%, and they were more sensitive to the osmotic pressures of NaCl, KCl and sorbitol. In gene-deleted mutants, the antagonistic activities to Botrytis cinerea, S. sclerotiorum and Fusarium oxysporum f. sp. cucumerinum significantly decreased (P<0.05), and their parasitic ability to S. sclerotiorum sclerotia dropped from 4^th of the wild type to 2^nd degree. In greenhouse, the control efficiencies against soybean Sclerotinia stem rot decreased by 50.5% compared to the wild type. However, after the gene CrSdr was complemented, the biocontrol activities of 67-1 were recovered, suggesting that CrSdr plays important roles in mycoparasitism and biological control of C. rosea. The research provides a foundation for understanding the mechanism of C. rosea mycoparasitism, and will be helpful for the development of highly efficient biocontrol fungal agents against plant diseases.

Key words: Clonostachys rosea, short-chain dehydrogenase, mycoparasitism, Sclerotinia sclerotiorum, gene knockout and complementation, real-time PCR

CLC Number:

S476

CHEN Yingying, WANG Yanan, Lü Binna, TAN Xiaodong, LI Shidong, SUN Manhong, MA Guizhen. Function of Short-chain Dehydrogenase Gene CrSdr in Clonostachys rosea 67-1[J]. Chinese Journal of Biological Control, 2021, 37(4): 749-760.

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