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Chinese Journal of Biological Control ›› 2021, Vol. 37 ›› Issue (4): 749-760.DOI: 10.16409/j.cnki.2095-039x.2021.06.001

• RESEARCH REPORTS • Previous Articles     Next Articles

Function of Short-chain Dehydrogenase Gene CrSdr in Clonostachys rosea 67-1

CHEN Yingying1,2, WANG Yanan1,2, Lü Binna2, TAN Xiaodong3, LI Shidong2, SUN Manhong2, MA Guizhen1   

  1. 1. Jiangsu Key Laboratory of Marine Bioresources and Eco-environment, Jiangsu Ocean University, Lianyungang 222000, China;
    2. Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
    3. Beijing Soil Fertilizer Workstation, Beijing 100029, China
  • Received:2021-03-16 Published:2021-09-18

Abstract: A differentially expressed short-chain dehydrogenase gene, CrSdr, was identified from the transcriptome of Clonostachys rosea 67-1 mycoparasitizing the sclerotia of Sclerotinia sclerotiorum, which was previously constructed in our lab. Quantitative real-time PCR detection showed that the expression level of CrSdr increased 4 times after the induction of sclerotia for 24 h. Gene knock-out and complementation were conducted to investigate the function of CrSdr. The results showed that the gene-deleted mutants had the same growth speed with the wild type, spore yields increased by 43.2%, and they were more sensitive to the osmotic pressures of NaCl, KCl and sorbitol. In gene-deleted mutants, the antagonistic activities to Botrytis cinerea, S. sclerotiorum and Fusarium oxysporum f. sp. cucumerinum significantly decreased (P<0.05), and their parasitic ability to S. sclerotiorum sclerotia dropped from 4th of the wild type to 2nd degree. In greenhouse, the control efficiencies against soybean Sclerotinia stem rot decreased by 50.5% compared to the wild type. However, after the gene CrSdr was complemented, the biocontrol activities of 67-1 were recovered, suggesting that CrSdr plays important roles in mycoparasitism and biological control of C. rosea. The research provides a foundation for understanding the mechanism of C. rosea mycoparasitism, and will be helpful for the development of highly efficient biocontrol fungal agents against plant diseases.

Key words: Clonostachys rosea, short-chain dehydrogenase, mycoparasitism, Sclerotinia sclerotiorum, gene knockout and complementation, real-time PCR

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