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Chinese Journal of Biological Control ›› 2021, Vol. 37 ›› Issue (6): 1241-1249.DOI: 10.16409/j.cnki.2095-039x.2021.06.023

• TECHNICAL REVIEWS • Previous Articles    

Study of Biocontrol Efficacy on Cucurbit Gummy Stem Blight by Lysobacter capsici NF87-2

ZUO Yang1, ZHANG Xinning2, QIAO Junqing1, LI Yuerong3, LIU Yongfeng1, LIU Youzhou1   

  1. 1. Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;
    2. Agriculture Committee of Suining County, Suining 221225, China;
    3. College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, China
  • Received:2021-07-17 Published:2021-12-15

Abstract: Cucurbit gummy stem blight caused by Didymella bryoniae is an important soil-borne fungal diseases and cause significant economic losses. In order to screen and evaluate the antagonistic bacteria against D. bryoniae, the antagonistic activity in vitro and control effect on cucurbit gummy stem blight in pot experiment were investigated in this study. Antagonistic examination against D. bryoniae in vitro revealed that Lysobacter capsici NF87-2 showed strong and steady antagonism, with the inhibition rate of 81.6%. Inhibition rates of strain NF87-2 and its secondary metabolites on spore germination of D. bryoniae were 59.4% and 67.2%, respectively. Seed germination percentage and seedling emergence rate were 83% and 82%, respectively, after inoculated with L. capsici NF87-2 and D. bryoniae together while 48% and 38%, respectively, after only inoculated with D. bryoniae. The fermented liquid and its secondary metabolite of strain NF87-2 could also effectively control gummy stem blight on cucumber in pot experiment, with the control efficiency of 81.6% and 66.5%, respectively. Therefore, strain NF87-2 was a prospective antagonistic bacterium and it is hopeful to be developed as a biological pesticide to control cucurbit gummy stem blight.

Key words: Didymella bryoniae, Lysobacter capsici NF87-2, inhibition rate, control effect

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