Screening and Optimization of Conditions for Cultivating Beauveria bassiana with Spent Fungus Substrates

doi:10.16409/j.cnki.2095-039x.2022.03.026

Abstract

Abstract: Beauveria bassiana is an entomogenous fungus showing significant control of agricultural and forestry insect pests.To further promote the development of B.bassiana pesticide,it is indispensable to develop low-cost B.bassiana fermentation methods or optimize its fermentation conditions.Using spent mushroom substrates as the main medium to cultivate B.bassiana,this study preliminarily optimized the composition of the culture medium and the conditions for cultivating B.bassiana by single-factor experiments and orthogonal experiments.The results showed that spent mushroom substrates were suitable as the main culture medium for B.bassiana.When the material-to-liquid ratio is 1:4,with addition of 0.3% manganese sulfate,0.5% ammonium nitrate,and 10% inoculation amount,at a cultivating temperature of 25℃ and a cultivating period of 4 d,the spore production of B.bassiana reached 2.0×10⁹ conidia/mL.The results confirm the feasibility of cultivating B.bassiana with spent mushroom substrates,which may shed light on low-cost fermentation of other biocontrol fungi.

Key words: Beauveria bassiana, spent fungus substrates, optimization of culture conditions

CLC Number:

S476.1

GAO Feng, CHENG Zhiqiang, YU Xiaobin. Screening and Optimization of Conditions for Cultivating Beauveria bassiana with Spent Fungus Substrates[J]. Chinese Journal of Biological Control, 2022, 38(5): 1184-1192.

References

[1] 李增智.我国利用真菌防治害虫的历史、进展及现状[J].中国生物防治学报, 2015, 31(5):699-711.
[2] Todd A, Ugine, Stephen P, et al. Microbial biological control potential of three strains of Beauveria bassiana s. l. against greenhouse shore fly Scatella tenuicosta:assessment of virulence, mass production capacity, and effects on shore fly reproduction[J]. Biological Control, 2013, 65(3):348-356.
[3] 陈孟,李英,王雪菲,等.大米固态发酵生产白僵菌孢子粉的条件优化[J].林业与生态科学, 2019, 34(4):414-418.
[4] 孙明,任巧云,关贵全,等.对蜱致病性球孢白僵菌培养条件的优化[J].微生物学通报, 2011, 38(7):1022-1030.
[5] 陈辰,朱润琪,臧晓静,等.烟梗废弃物固态发酵生产白僵菌的适宜条件筛选[J].烟草科技, 2018, 51(7):24-29.
[6] 陈辰,朱润琪,臧晓静,等.利用废弃烟梗发酵生产白僵菌的响应面优化[J].中国烟草科学, 2018, 39(2):46-53.
[7] 樊金华,薛皎亮,谢映平,等.布氏白僵菌液体培养条件的优化研究[J].山西大学学报(自然科学版), 2013, 36(2):271-274.
[8] 张亚平,吴圣勇,王海鸿,等.球孢白僵菌液体生长营养和培养条件优化[J].植物保护, 2014, 40(6):87-93.
[9] 胡加付,李农昌,樊美珍,等.白僵菌固态发酵试验研究[J].微生物学通报, 2005(6):20-25.
[10] 张亚平.防治蓟马的白僵菌营养和发酵条件研究[D].北京:中国农业科学院, 2014.
[11] 张莹,田龙,徐敏慧,等.食用菌菌糠综合利用研究进展[J].微生物学通报, 2020, 47(11):3658-3670.
[12] 杨和川,谭一罗,苏文英,等.食用菌菌糠资源化利用研究进展[J].农业工程, 2018, 8(10):54-58.
[13] 程志强,刘景华,康立娟,等.废弃黑木耳菌糠特征及环境影响分析[J].环境污染与防治, 2012, 34(7):45-48, 54.
[14] 杨鹏,韩建东,万鲁长,等.利用杏鲍菇菌渣栽培平菇试验[J].中国食用菌, 2015, 34(5):14-16.
[15] Journal P, Yuliana T, Putri N Z, et al. Study of Ganoderma lucidum in Laccase production using corncob and paddies straw substrates on submerged fermentation system[J]. Pakistan Journal of Biological Sciences, 2020, 23(8):1060-1065.
[16] Musatti A, Ficara E, Mapelli C, et al. Use of solid digestate for lignocellulolytic enzymes production through submerged fungal fermentation[J]. Journal of Environmental Management, 2017, 199(1):1-6.
[17] 叶淑芬,窦森,张一枫,等.里氏木霉和哈茨木霉协作方式对玉米秸秆形成类腐殖质的影响[J].吉林农业大学学报, DOI:10.13327/j.jjlau. 2021.1281.
[18] 黄欣.基于咖啡渣基料的生防菌剂发酵及其在连作障碍土壤修复中的应用研究[D].扬州:扬州大学, 2020.
[19] Agale S V, Gopalakrishnan S, Ambhure K G, et al. Mass production of entomopathogenic fungi (Metarhizium anisopliae) using different grains as a substrate[J]. International Journal of Current Microbiology and Applied Sciences, 2018, 7(1):2227-2232.
[20] Mok W K, Yong X T, Xiao M L, et al. Effects of submerged liquid fermentation of Bacillus subtilis WX-17 using okara as sole nutrient source on the composition of a potential probiotic beverage[J]. Food Science& Nutrition, 2020, 8:3119-3127.
[21] 刘世祥,张荣胜,刘永锋,等.深绿木霉TA-9分生孢子固体发酵的响应曲面法优化[J].农药学学报, 2022, 24(1):96-104.
[22] Rezende L, Carvalho A, Costa L B, et al. Optimizing mass production of Trichoderma asperelloides by submerged liquid fermentation and its antagonism against Sclerotinia sclerotiorum[J]. World Journal of Microbiology and Biotechnology, 2020, 36(113):112.
[23] 刘琪,耿丽丽,刘春琴,等.提升Bt和球孢白僵菌防治蛴螬效果的颗粒载体[J].中国生物防治学报, 2021, 37(2):193-200.
[24] 孙明,任巧云,漆晶晶,等.球孢白僵菌对小亚璃眼蜱的毒力测定及其孢外水解酶活性分析[J].中国动物检疫, 2021, 38(12):116-120.
[25] 葛银银,王滨.湿度对球孢白僵菌和玫烟色虫草田间防治棉蚜效果的影响[J].中国生物防治学报, 2022, 38(1):172-179.
[26] 刘柳.菌糠复合肥的研制及其在草莓种植中的应用[D].沈阳:沈阳大学, 2018.
[27] 赵文瑜.香菇菌糠有机肥在设施果蔬生产上的应用[D].南京:南京农业大学, 2018.