Isolation and Identification of Three Beauveria bassiana Isolates and Their Virulence against Mythimna separata under Laboratory Condition

doi:10.16409/j.cnki.2095-039x.2022.03.006

Abstract

Abstract: Three entomopathogenic fungi strains were isolated from soil samples collected from Hebei Province using Tenebrio molitor larvae as bait and were identified through morphological characteristics and rDNA sequence analysis. Results showed that the three strains were Beauveria bassiana, named Z-G2-5, M-G4 and M-G3, respectively. Bioassay showed that all the three B. bassiana strains could infect the larvae of Mythimna separata effectively, but showed varying virulence to the 3^rd instar larvae of M. separata. At 5×10⁸ spores/mL, the strain Z-G2-5 caused a peak cumulative corrected mortality of 96.77% at the 6^th day post infection, with a LT₅₀ of 3.02 d, a LC₅₀ of 4.95×10⁶ spores/mL. At the same concentration, the cumulative mortality with M-G3 and M-G4 strains were only 66.67% and 71.11%, respectively, the LT₅₀ values were 4.89 d and 3.97 d, respectively, and the LC₅₀ values were 1.18×10⁸ spores/mL and 1.13×10⁷ spores/mL, respectively. The results indicated that, compared with the other two strains, the strain Z-G2-5 had stronger pathogenicity and faster lethal rate to armyworm larvae, and it can be used as a candidate strain with potential application for biological control of M. separata.

Key words: Beauveria bassiana, Mythimna separata, pathogenicity, biological control, identification

CLC Number:

S476.1

CHENG Yin, ZHENG Jiyang, WANG Dun. Isolation and Identification of Three Beauveria bassiana Isolates and Their Virulence against Mythimna separata under Laboratory Condition[J]. Chinese Journal of Biological Control, 2022, 38(2): 521-530.

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