Sequence Structure and Spatiotemporal Expression Analysis of AKT, MAPK and RPS6KB Genes of Insulin Signaling Pathway in Propylea japonica

doi:10.16409/j.cnki.2095-039x.2022.11.008

Abstract

Abstract: To determine the sequence structure and spatiotemporal expression patterns of AKT, MAPK and RPS6KB genes in the insulin signaling pathway of Propylea japonica, the full-length genes of AKT, MAPK and RPS6KB were acquired from P. japonica genome. Protein sequence structure analysis showed that the genes encode 522, 332, and 375 amino acids, respectively. Expression profiles of the genes in different developmental stages (1, 3, 5, 7 days after eclosion) and tissues (head, thorax, abdomen, intestine) of P. japonica female adults were investigated through the method of real-time quantitative PCR. The results showed that AKT, MAPK and RPS6KB were significantly up-regulated in the 7-d old females and reached the highest level. AKT was highly expressed in the head in the 1-d and 3-d old females, and highly expressed in the intestine and thorax in the 5-d and 7-d old females, respectively. The expression level of MAPK gene in the head was significantly higher than that in the thorax, abdomen and intestine in the 1-d, 3-d, 5-d and 7-d old females. Expression level of RPS6KB gene was the highest in abdomen in the1-d, 3-d, 5-d and 7-d old females, and significantly higher than that in head, thorax and intestine. Taken together, the expression levels of AKT, MAPK and RPS6KB genes differ in different developmental stages and tissues of P. japonica, which serves as a foundation for further study on the role of insulin signaling pathway in reproductive development of P. japonica..

Key words: Propylea japonica, insulin signaling pathway, spatiotemporal expression pattern, qRT-PCR

CLC Number:

S476.2

CHEN Lulu, HUANGFU Ningbo, ZHAO Likang, ZHANG Kaixin, LI Dongyang, JI Jichao, MA Deying, CUI Jinjie. Sequence Structure and Spatiotemporal Expression Analysis of AKT, MAPK and RPS6KB Genes of Insulin Signaling Pathway in Propylea japonica[J]. Chinese Journal of Biological Control, 2023, 39(2): 280-288.

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