Influence of Three Pesticides on the Expression of Six P450 Genes in Propylea japonica

doi:10.16409/j.cnki.2095-039x.2021.11.020

Abstract

Abstract: The potential effects of commonly used fungicides, herbicides and plant growth regulators (thiophanate-methyl, quizalofop-p-ethyl, and mepiquat chloride) on Propylea japonica were measured by investigating the fertility, survival rate and the transcriptional response of detoxification enzymes. Interestingly, fecundity and survival rate of P. japonica showed no significant changes after the insects were treated with thiophanate-methyl, quizalofop-ethyl, or mepiquat chloride. Six P450 genes (CYP307A1, CYP314A1, CYP345D2, CYP6BQ22, CYP9Z1, CYP9Z4) were identified from the P. japonica genome. The protein sequence structure analysis revealed that the genes encode 510, 421, 353, 314, 343, 385 amino acids, respectively, and all contain Pfam P450 domain. The P450 genes CYP307A1, CYP314A1, CYP345D2 belong to the CYP3 family, the CYP9Z1 and CYP9Z4 belong to the CYP9 family, and CYP6BQ22 belongs to the CYP6 family. The real-time fluorescent quantitative PCR technology was used to detect the transcriptional responses of the genes in P. japonica to the treatment by the agricultural agents. The results showed that thiophanate-methyl treatment significantly up-regulated the expression levels of CYP307A1 and CYP6BQ22, quizalofop-p-ethyl stress significantly up-regulated the expression of CYP307A1, CYP345D2, and CYP6BQ22 while significantly down-regulated the expression of CYP9Z1 and CYP9Z4. The treatments with mepiquat chloride significantly up-regulated expression of CYP307A1 and CYP9Z1. Although no significant biological variations were observed in P. japonica treated with the three pesticides, several P450 genes expressions were up, which might contribute to the detoxification metabolic effect of P. japonica. This study provides important evidence for exploring the potential influences of agricultural agents on P. japonica.

Key words: Propylea japonica, cytochrome P450, thiophanate-methyl, quizalofop-p-ethyl, mepiquat chloride

CLC Number:

S476

CHANG Guofeng, XUE Hui, HUANGFU Ningbo, ZHANG Kaixin, LI Dongyang, WANG Lisha, JI Jichao, MA Weihua. Influence of Three Pesticides on the Expression of Six P450 Genes in Propylea japonica[J]. Chinese Journal of Biological Control, 2022, 38(6): 1449-1458.

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