Control Effect of Trichogramma chilonis carrying Beauveria bassiana on Soybean Podborer

doi:10.16409/j.cnki.2095-039x.2023.01.008

Abstract

Abstract: Soybean moth is an important pest causing huge economic loss in the main soybean producing areas in China. Beauveria bassiana and Trichogramma are the two important biological control agents against soybean moth. However, how to combine the two agents to realize sustainable control of soybean moth is not addressed. Herein, three B. bassiana strains with high virulence against the pest were isolated, identified and screened from the diseased soybean moth larvae. The median lethal time (LT₅₀) of the strains to the mature larvae of the pest were 6.21 days, 6.39 days and 6.87 days, respectively. The B. bassiana conidia and their highly adsorptive adjunct, 0.1% (w/v) starch solution, showed no effect on the emergence and parasitism of pest eggs of T. chilonis. With the adjunct, T. chilonis was able to carry fungal conidia of 3.56×10⁴ per wasp. The two-year field experiments showed that, compared with Trichogramma alone, the pest control effect of Trichogramma carrying B. bassiana was increased by 11.80% and 8.31%, respectively, to 70.44% and 64.75%, respectively, thereby improving the pest control efficiency and realizing synergy without increasing the control cost.

Key words: Leguminivora glycinivorella, biocontrol microorganism, natural enemy, synergy

CLC Number:

S476

ZHAO Yu, LIU Jia, LU Yang, SUI Li, XU Wenjing, DU Qian, LI Qiyun, ZHANG Zhengkun. Control Effect of Trichogramma chilonis carrying Beauveria bassiana on Soybean Podborer[J]. Chinese Journal of Biological Control, 2023, 39(4): 760-766.

References

[1] 付晓霞, 徐伟, 卢爱军, 等. 大豆植株挥发物组分变化与大豆食心虫为害的关系[J]. 吉林农业大学学报, 2014, 36(4):389-394.
[2] 徐伟, 张益恺, 董亚南, 等. 不同品种大豆蛋白酶抑制剂活性及其对大豆食心虫幼虫的影响[J]. 吉林农业大学学报, 2019, 41(3):267-273.
[3] 罗宝君. 不同赤眼蜂蜂种对大豆食心虫的防治效果[J]. 安徽农业科学, 2017, 45(12):141-143.
[4] 李启云, 路杨, 隋丽, 等. 植物保护与农业绿色发展[J]. 吉林农业大学学报, 2021, 43(1):9-15.
[5] 宋丽丽, 丛林, 张燕如, 等. 豆科种实害虫生物防治研究进展[J]. 中国农学通报, 2021, 37(10):113-120.
[6] Smith S M. Biological control with Trichogramma:advances, success, and potential of their use[J]. Annual Review of Entomology, 1996, 41:1, 375-406.
[7] 陈日瞾, 石钟锋, 范莉莉, 等. 载菌赤眼蜂生物学特性及其对亚洲玉米螟防治效果的初步研究[J]. 吉林农业大学学报, 2007, 29(3):259-261.
[8] 杨芷, 路杨, 毛刚, 等. 松毛虫赤眼蜂携带球孢白僵菌防治亚洲玉米螟技术研究与应用[J]. 中国生物防治学报, 2020, 36(1):52-57.
[9] Wang P, Li M J, Bai Q R, et al. Performance of Trichogramma japonicum as a vector of Beauveria bassiana for parasitizing eggs of rice striped stem borer, Chilo suppressalis[J]. Entomologia Generalis, 2021, 41:147-155.
[10] 林英, 宋丽威, 臧连生, 等. 三种本地赤眼蜂对大豆食心虫卵的寄生潜能[J]. 中国农业科学, 2014, 47(19):3810-3816.
[11] 常玉明, 张正坤, 赵宇, 等. 玉米大豆间作对玉米主要病虫害发生及其产量的影响[J]. 植物保护学报, 2021, 48(2):332-339.
[12] 李乐, 路杨, 赵宇, 等. 球孢白僵菌生物学性状与对玉米螟毒力相关性分析[J]. 玉米科学, 2021, 29(1):162-169.
[13] Arseneau J R, Steeves R, Laflamme M. Modified low-salt CTAB extraction of high-quality DNA from contaminant-rich tissues[J]. Molecular Ecology Resources, 2017, 17(4):686-93.
[14] 赵宇, 张佳诗, 刘艳微, 等. 创制转Mad1基因球孢白僵菌工程菌株提高对玉米螟毒力[J]. 玉米科学, 2019, 27(5):39-44, 51.
[15] 李美君, 白庆荣, 臧连生, 等. 水稻二化螟病原真菌鉴定及其致病力[J]. 中国生物防治学报, 2019, 35(1):63-69.
[16] 孙艺昕, 门兴元, 李超, 等. 双委夜蛾高致病力球孢白僵菌的筛选及在玉米内定殖率的测定[J]. 中国生物防治学报, 2019, 35(1):70-74.
[17] 施泽升, 陈海生, 覃振强, 等. 崇左甘蔗螟虫种群动态及螟黄赤眼蜂防治效果评价[J]. 中国生物防治学报, 2018, 34(5):11-17.
[18] Blibech I, Ksantini M, Jardak T, et al. Effect of insecticides on Trichogramma parasitoids used in biological control against Prays oleae insect pest[J]. Advances in Chemical Engineering and Science, 2015, 5:362-372.
[19] 李旋, 崔娟, 秦昊东, 等. 大豆食心虫田间性诱监测与防治指标研究[J]. 中国油料作物学报, 2020, 42(4):701-707.
[20] 严森, 任小云, 王登杰, 等. 昆虫病原真菌在害虫防治中对天敌生物的影响研究进展[J]. 中国生物防治学报, 2023, 39(1):221-230.
[21] 孙光芝, 张俊杰, 阮长春, 等. 赤眼蜂载菌方式筛选及田间防治玉米螟效果[J]. 吉林农业大学学报, 2004, 26(2):138-141.
[22] Potrich M, Alves L F A, Lozano E, et al. Interactions between Beauveria bassiana and Trichogramma pretiosumunder laboratory conditions[J]. Entomologia Experimentalis et Applicata, 2015, 154(3):213-221.
[23] 杨芷, 路杨, 张庆贺, 等. 利用荧光观测明确载球孢白僵菌松毛虫赤眼蜂对亚洲玉米螟的防控增效作用[J]. 中国生物防治学报, 2020, 36(1):58-64.
[24] 徐庆丰, 冯真, 马淑丽. 应用白僵菌Beauveria bassiana (Bals.) Vuill.防治大豆食心虫Grapholitha glycinivorella Mats.的初步研究[J]. 昆虫学报, 1959(3):203-217.
[25] 程媛, 韩岚岚, 于洪春, 等. 性诱剂、赤眼蜂和化学药剂协同防治大豆食心虫的研究[J]. 应用昆虫学报, 2016, 53(4):752-758.
[26] 温玄烨, 宋丽威, 许晶, 等. 三种赤眼蜂对不同密度大豆食心虫卵的寄生反应及其自身密度的干扰效应[J]. 植物保护学报, 2015, 42(4):626-631.
[27] 杨旭. 黏虫赤眼蜂和松毛虫赤眼蜂对柞蚕卵的共寄生及协同繁育研究[D]. 长春:吉林农业大学, 2016.
[28] Zhang Z K, Lu Y, Xu W J, et al. Influence of genetic diversity of seventeen Beauveria bassiana isolates from different hosts on virulence by comparative genomics[J]. BMC Genomics, 2020, 21:451.
[29] Zhang Z K, Lu Y, Xu W J, et al. RNA sequencing analysis of Beauveria bassiana isolated from Ostrinia furnacalis identifies the pathogenic genes[J]. Microbial Pathogenesis, 2019, 130:190-195.
[30] 陶淑霞, 陆子慧. 大豆食心虫高毒力球孢白僵菌菌株的筛选[J]. 江苏农业科学, 2018, 46(5):91-93.